Influenza A nucleoprotein Antibody (010) [PE]

• Reactivity: V
• Applications: WB, ELISA
• Clone: 010
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: PE

Influenza A nucleoprotein Antibody (010) [PE] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Influenza A H3N2 (Uniprot#: P22435.2; Met1-Asn498).
• Specificity: Widely recognize Influenza A Nucleoprotein
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Pan Influenza A

Has cross-reactivity in ELISA with:
H1N1 (A/Brevig Mission/1/1918) NP
H1N1 (A/Brisbane/02/2018) NP
H1N1 (A/California/07/2009) NP
H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP
H1N1 (A/Hawaii/70/2019) NP
H1N1 (A/Michigan/45/2015 NP
H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M)
H1N1 (A/Victoria/2570/2019)/(A/Wisconsin/588/2019) NP
H2N2 (A/Ann Arbor/6/1960) NP
H3N2 (A/Aichi/2/1968) NP
H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP
H3N2 (A/Hong Kong/1/1968) NP
H3N2 (A/Hong Kong/2671/2019) NP
H3N2 (A/Hong Kong/45/2019) NP
H3N2 (A/Hong Kong/4801/2014) NP
H3N2 (A/Kansas/14/2017) NP
H3N2 (A/Switzerland/9715293/2013) NP
H7N9 (A/Anhui/1-BALF_RG6/2013) NP
H7N9 (A/Shanghai/2/2013) NP
Influenza B (B/Phuket/3073/2013) NP
Influenza B (B/Brisbane/60/2008) NP
Influenza B (B/Colorado/06/2017) NP

No cross-reactivity in ELISA with:
Influenza B (B/Florida/4/2006) NP
Influenza B (B/Washington/02/2019) NP
SARS-CoV-2 Nucleocapsid Protein
HCoV-229E Nucleocapsid Protein
HCoV-NL63 Nucleocapsid Protein
HCoV-HKU1 Nucleocapsid Protein
HCoV-OC43 Nucleocapsid Protein

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for Influenza A nucleoprotein Antibody (010) [PE]

• Influenza A virus NP
• NP
• NP-A
• Nucleocapsid protein
• Nucleoprotein

Background

The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.