Inhibin beta A Antibody (CL15357) [Unconjugated] - BSA Free

Analysis in human placenta and kidney tissues using NBP3-43663 antibody. Corresponding INHBA RNA-seq data are presented for the same tissues.

Staining of human prostate shows no positivity in glandular cells as expected.

Staining of rat cerebral cortex shows moderate cytoplasmic positivity in a subset of neurons in the upper layers.

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in a subset of neurons.

Staining of human kidney shows no positivity in cells in tubules as expected.

Analysis in human cell line U-138MG.

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: CL15357
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Inhibin beta A Antibody (CL15357) [Unconjugated] - BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P08476, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: INHBA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Inhibin beta A Antibody (CL15357) [Unconjugated] - BSA Free

• Activin Beta A
• Activin beta-A chain
• beta A (activin A, activin AB alpha polypeptide)
• EDF
• erythroid differentiation factor
• Erythroid differentiation protein
• follicle-stimulating hormone-releasing protein
• FRP
• FSH-releasing protein
• INHBA
• inhibin beta A chain
• Inhibin Beta A
• Inhibin Subunit Beta A
• inhibin, beta A
• Inhibin, beta-1

Background

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INHBA