InnovaCoat® GOLD 40nm Mini Antibody Labeling Kit

InnovaCoat GOLD 40nm Mini Antibody Labeling Kit [230-0010]

Lateral Flow Assay: InnovaCoat GOLD 40nm Mini Antibody Labeling Kit [230-0010] - A comparison between the percentage binding of anti-cortisol antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat® technology. All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.

Lateral Flow Assay: InnovaCoat GOLD 40nm Mini Antibody Labeling Kit [230-0010] - A comparison between the signal intensity, measured on a commercially available lateral flow strip reader, for anti-IgA antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat® technology. All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.

Lateral Flow Assay: InnovaCoat GOLD 40nm Mini Antibody Labeling Kit [230-0010] - A comparison between the signal intensity, measured on a commercially available lateral flow strip reader, for anti-IgA antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat® technology. All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.

Product Discontinued

InnovaCoat® GOLD 40nm Mini Antibody Labeling Kit Summary

• Description: InnovaCoat GOLD antibody labeling kits enable the covalent attachment of antibodies, proteins, peptides and other biomolecules to gold nanoparticles with a proprietary InnovaCoat coating. The hands-on time is around 30 seconds and the gold conjugate is ready to use after 20 minutes.
  
  

  Features	Benefits
  Quick and easy to use	Save time, no special knowledge required
  Different nanoparticle sizes, concentrations and chemistries available	Flexible
  Freeze dried	Ships at ambient temperature, long shelf-life
  Fully scalable	Easy transfer from R&D to manufacturing
  Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
  Proprietary surface coating	Covalent bond, stable conjugates formed
  Covalent conjugation uses significantly less antibody than passive conjugation	Save precious antibody
  Uniform spherical shape and narrow size distribution	Batch-to-batch consistency

• Kit Type: Antibody Labeling Kit

Applications/Dilutions

Publications

Read Publications using 230-0010.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

InnovaCoat GOLD® is a registered trademark of Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for InnovaCoat (R) GOLD 40nm Mini Kit (230-0010)(7)

Publications using 230-0010

• Ben Aissa A, Jara JJ, Sebastian RM et al. Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens. Biosens Bioelectron. 2017-02-15 [PMID: 27599431]
• Lee S, O'Dell D, Hohenstein J et al. NutriPhone: a mobile platform for low-cost point-of-care quantification of vitamin B12 concentrations Sci Rep. 2016-06-15 [PMID: 27301282]
• Ortega Arroyo J, Cole D, Kukura P Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging. Nat Protoc. 2016-01-01 [PMID: 26938114]
• Cabezon I, Manich G, Martin-Venegas R et al. Trafficking of gold nanoparticles coated with the 8D3 anti-transferrin receptor antibody at the mouse blood-brain barrier. Mol Pharm. 2015-11-02 [PMID: 26440359]
• Holstein CA, Chevalier A, Bennett S et al. Immobilizing affinity proteins to nitrocellulose: a toolbox for paper-based assay developers. Anal Bioanal Chem. 2016-01-01 [PMID: 26427504]
• Ching KH, He X, Stanker LH et al. Detection of Shiga Toxins by Lateral Flow Assay. Toxins (Basel). 2015-04-03 [PMID: 25855129]
• Wang HK, Tsai CH, Chen KH et al. Cellulose-Based Diagnostic Devices for Diagnosing Serotype-2 Dengue Fever in Human Serum. Adv Healthc Mater. 2013-01-01 [PMID: 23843297] (FLOW)

FAQs for InnovaCoat (R) GOLD 40nm Mini Kit (230-0010). (Showing 1 - 7 of 7 FAQs).

1. Can antibodies from different species be used?
   • Yes, the InnovaCoat® system has been tested with antibodies from a variety of species including mouse, rabbit, goat and sheep.
2. Can antibody fragments be conjugated?
   • Yes. One of the advantages of the protective coat is that it is less likely than a bare metal surface to cause denaturation and loss of affinity of the antibody fragments.
3. Does the antibody bind to the metal surface?
   • No. The protective surface coat completely shields the metal surface and prevents direct metal-antibody interactions. For this reason, you cannot use InnovaCoat® GOLD for passive conjugation of antibodies.
4. Is the kit suitable for conjugating analytes and other small molecules?
   • Yes, but please contact Innova technical services. Depending on the functional groups on your analyte you may need a different type of surface or different chemistry for optimal results.
5. What can I do if my antibody preparation contains interfering substances
   • The simplest procedure is to dialyse the antibody against a suitable buffer. Relatively weak buffers (e.g. 20mM) are preferred so that the pH conditions of the covalent reaction are not significantly altered upon addition of the antibody. See also the comments in Section 3 of the protocol. If dialysis is not practical you can use other popular methods of buffer exchange if required e.g. desalting columns.
6. What if I need bulk material?
   • The kit that you have purchased is a convenience product for rapid production of small quantities of conjugate for screening purposes. Bulk quantities of InnovaCoat® GOLD can be provided in a variety of formats. Please enquire.
7. What type of linkage to gold is formed?
   • The antibody becomes covalently and irreversibly attached via lysine residues to the InnovaCoat® surface.