![INSTA-Blot Multi-species Brain,Testis and Ovary [NBP2-30115] - Approximately 20 ug per lane of tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. human brain; 3. mouse brain; 4. rat brain; 5. Molecular weight marker; 6. human testis; 7. mouse testis; 8. rat testis; 9. Molecular weight marker; 10. human ovary; 11. mouse ovary; 12. rat ovary.](http://images.novusbio.com/fullsize/INSTA-Blot-Multi-species-Brain-Testis-and-Ovary-NBP2-30115-img0002.jpg "INSTA-Blot Multi-species Brain,Testis and Ovary [NBP2-30115] - Approximately 20 ug per lane of tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. human brain; 3. mouse brain; 4. rat brain; 5. Molecular weight marker; 6. human testis; 7. mouse testis; 8. rat testis; 9. Molecular weight marker; 10. human ovary; 11. mouse ovary; 12. rat ovary.")
| Reactivity | HuSpecies Glossary |
| Description | Ready-to-use PVDF membrane with 11 human, mouse and rat tissue lysates (20 ug total protein per lane). |
| Background | INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Multi-species Brain, Testis and Ovary NBP2-30115 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDSPAGE and electr...o-blotting steps. With INSTA-Blot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop. Show More |
| Preparation Method |
The INSTA-Blot™ Multi-species Brain, Testis and Ovary NBP2-30115 contains denatured proteins from tissue lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is provided ready-to-use in your western blot protocol. |
| Application Notes | Human tissues are from donors with no known disease. Mouse tissues are from Balb/c mice. Rat tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). |
|
| Publications |
|
| Storage | Store at room temperature. |
| Publication using NBP2-30115 | Applications | Species |
|---|---|---|
| Flaumenhaft Robert. alpha-granules: a story in the making. Blood. 2012-12-13 [PMID: 23243155] (Human) | Human |
