Integrin alpha 1/CD49a Antibody (639508)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Integrin a1/CD49a Monoclonal Antibody (Catalog # MAB5676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a1/CD49a at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Levels of HAV RNA at 24 hpi in H1-HeLa cells engineered by CRISPR/Cas9 to be knocked-out for the indicated integrins inoculated with naked HAV (mean ± SD).Immunoblots of whole-cell lysates harvested from the indicated cell lines. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30801249), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clone: 639508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 1/CD49a Antibody (639508) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived human Integrin alpha 1/CD49a
  Phe29-Pro1141
  Accession # P56199
• Specificity: Detects human Integrin alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rhIntegrin alpha 2, 3, 4, 5, 6, 8, 9, 10, 11, L, M, V, X, alpha 6x1/ beta 4, alpha D/ beta 2, alpha 7x2/ beta 1, alpha E/ beta 7, or alpha 2b/ beta 3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 1/CD49a Antibody (639508)

• CD49 antigen-like family member A
• CD49a antigen
• CD49a
• Integrin alpha 1
• integrin, alpha 1
• ITGA1
• Laminin and collagen receptor
• very late activation protein 1
• VLA-1
• VLA1integrin alpha-1

Background

Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210 kDa member of the integrin alpha chain family of molecules.  It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium.  Integrin alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI.  It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus.  Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail.  The ECD contains one vWFA/I-domain (aa 147-360) that binds collagen, plus multiple divalent cation binding sites.  Potential splice variants exist that show a two and four aa substitution for aa 765-1179.  Over aa 29-1141 (the ECD), human Integrin alpha 1 shares 88% aa identity with mouse Integrin alpha 1. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 1/CD49a Antibody (MAB5676)(6)

Publications using MAB5676

• Yen-Lin Huang, Ching-Yeu Liang, Danilo Ritz, Ricardo Coelho, Dedy Septiadi, Manuela Estermann et al. Collagen-rich omentum is a premetastatic niche for integrin alpha 2-mediated peritoneal metastasis eLife 10/7/2020 [PMID: 33026975]
• LB Gong, C Zhang, RX Yu, C Li, YB Fan, YP Liu, XJ Qu FKBP10 Acts as a New Biomarker for Prognosis and Lymph Node Metastasis of Gastric Cancer by Bioinformatics Analysis and in Vitro Experiments Onco Targets Ther, 2020-07-28;13(0):7399-7409. 2020-07-28 [PMID: 32801763] (Western Blot, Human)
• C Zeltz, J Alam, H Liu, PM Erusappan, H Hoschuetzk, A Molven, H Parajuli, E Cukierman, DE Costea, N Lu, D Gullberg α11?1 Integrin is Induced in a Subset of Cancer-Associated Fibroblasts in Desmoplastic Tumor Stroma and Mediates In Vitro Cell Migration Cancers (Basel), 2019-06-01;11(6):. 2019-06-01 [PMID: 31159419] (Western Blot, Human)
• Hongfei Yan, Chunlei Zheng, Zhi Li, Bowen Bao, Bowen Yang, Kezuo Hou et al. <p>NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer</p> Cancer Management and Research 5/3/2019 [PMID: 31043800]
• Rivera-Serrano EE, Gonzalez-Lopez O, Das A, Lemon SM. Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses Elife 2/25/2019 [PMID: 30801249]
• WS Chen, Z Cao, S Sugaya, MJ Lopez, VG Sendra, N Laver, H Leffler, UJ Nilsson, J Fu, J Song, L Xia, P Hamrah, N Panjwani Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3 Nat Commun, 2016-04-12;7(0):11302. 2016-04-12 [PMID: 27066737] (Western Blot, Human)

Review for Integrin alpha 1/CD49a Antibody (MAB5676) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 04/06/2022

Summary

• Application: Western Blot
• Sample Tested: HeLa cells
• Species: Human
• Lot: 639508

Bioinformatics

• Gene Symbol: ITGA1
• Uniprot:
  • P56199()