Integrin alpha 3/CD49c Antibody (IA3) [Unconjugated]

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 3/CD49c Monoclonal Antibody (Catalog # MAB1345, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Integrin a3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin a3/CD49c Monoclonal Antibody (Catalog # MAB1345) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: IA3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Integrin alpha 3/CD49c Antibody (IA3) [Unconjugated] Summary

• Immunogen: Human milk epithelial cell line
• Specificity: Detects human Integrin alpha 3/CD49c.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 3/CD49c Antibody (IA3) [Unconjugated]

• antigen identified by monoclonal J143
• CD49 antigen-like family member C
• CD49c antigen
• CD49c
• FLJ34631
• FLJ34704
• FRP-2
• Galactoprotein B3
• GAP-B3
• GAPB3CD49C
• Integrin alpha 3
• integrin alpha-3
• integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• ITGA3
• MSK18
• VCA-2
• very late activation protein 3 receptor, alpha-3 subunit
• VL3A
• VLA-3 subunit alpha
• VLA3a

Background

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8).

1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
8. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 3/CD49c Antibody (MAB1345)(7)

Publications using MAB1345

• Lin, LL;Nayak, B;Osmulski, PA;Wang, E;Wang, CP;Valente, PT;Wang, CM;Tan, X;Santanam, N;Wang, TL;Gaczynska, ME;Kost, ER;Huang, TH;Kirma, NB; PAI-1 uncouples integrin-?1 from restrain by membrane-bound ?-catenin to promote collagen fibril remodeling in obesity-related neoplasms Cell reports 2024-07-23 [PMID: 39046873] (Functional Assay, Human)
• Tania Baltazar, Bo Jiang, Alejandra Moncayo, Jonathan Merola, Mohammad Z. Albanna, W. Mark Saltzman et al. 3D bioprinting of an implantable xeno‐free vascularized human skin graft Bioengineering & Translational Medicine 2023-01-01 [PMID: 36684084]
• C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family PLoS ONE, 2022-09-15;17(9):e0274667. 2022-09-15 [PMID: 36108060] (Flow Cytometry, IHC, Human)
• Sally Dabelsteen, Emil M.H. Pallesen, Irina N. Marinova, Mathias I. Nielsen, Maria Adamopoulou, Troels B. Rømer et al. Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model Developmental Cell 2020-09-14 [PMID: 32710848]
• TL Tremblay, JJ Hill Biotin-transfer from a trifunctional crosslinker for identification of cell surface receptors of soluble protein ligands Sci Rep, 2017-04-19;7(0):46574. 2017-04-19 [PMID: 28422167] (Functional Assay, Human)
• Seltmann K, Cheng F, Wiche G, Eriksson J, Magin T Keratins Stabilize Hemidesmosomes through Regulation of beta4-Integrin Turnover. J Invest Dermatol, 2015-02-10;135(6):1609-20. 2015-02-10 [PMID: 25668239] (Flow Cytometry, Human)
• Turner NJ, Murphy MO, Kielty CM Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study. Circulation, 2006-08-14;114(8):820-9. 2006-08-14 [PMID: 16908762] (Neutralization, Human)

Bioinformatics

• Gene Symbol: ITGA3