Integrin alpha 4 beta 7/LPAM-1 Antibody (111D9.03) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Integrin alpha 4 beta 7/LPAM-1 Antibody (111D9.03) [DDX1434P-100] - Gut sections (Crohn's disease).

Flow Cytometry: Integrin alpha 4 beta 7/LPAM-1 Antibody (111D9.03) [DDX1434P-100] - FACS staining of RPMI 8866 cells (alpha4+beta7+) and HEK transfected cells with DDX1434(111D9) monoclonal antibody.

• Reactivity: Hu, Po
• Applications: Flow, ICC/IF, Binding Activity, ChIP
• Clone: 111D9.03
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Integrin alpha 4 beta 7/LPAM-1 Antibody (111D9.03) - Azide and BSA Free Summary

• Immunogen: rhua4b7 (R&D systems) and RPMI 8866 cells
• Isotype: IgA
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA4
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Chromatin Immunoprecipitation (ChIP)
  • Flow (Cell Surface)
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:33809123).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl pH 8
• Preservative: No Preservative
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Integrin alpha 4 beta 7/LPAM-1 Antibody (111D9.03) - Azide and BSA Free

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49d/beta7
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4 beta 7
• integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• LPAM1
• LPAM-1
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha

Background

The lymphocyte Peyer's patch adhesion molecule (LPAM-1), also known as integrin alpha4beta7, is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha4beta7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM-1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria. (1-4) The monoclonal antibody DATK32 recognizes a conformational epitope on the heterodimer. (1,2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 4 beta 7/LPAM-1 Antibody (DDX1434P-100)(3)

Publications using DDX1434P-100

• Biki Gupta, Ravi Prakash Rai, Pabitra B. Pal, Daniel Rossmiller, Sudrishti Chaudhary, Anna Chiaro, Shannon Seaman, Aatur D. Singhi, Silvia Liu, Satdarshan P. Monga, Smita S. Iyer, Reben Raeman, Alessandra Mangia, Maria Guido Selective Targeting of alpha 4 beta 7 /MAdCAM-1 Axis Suppresses Fibrosis Progression by Reducing Proinflammatory T Cell Recruitment to the Liver Cells 2024-04-27 [PMID: 38727292]
• Gupta B, Rai RP, Pal PB et al. Selective targeting of ? 4 ? 7 /MAdCAM-1 axis suppresses fibrosis progression in chronic liver disease bioRxiv : the preprint server for biology 2023-02-21 [PMID: 36865167] (IHC-Fr, Human)
• Yuan C, Li Y, Zhang E et al. The Mechanism of PEDV-Carrying CD3+ T Cells Migrate into the Intestinal Mucosa of Neonatal Piglets Viruses 2021-03-12 [PMID: 33809123] (FLOW, Porcine)

Bioinformatics

• Gene Symbol: ITGA4