Integrin alpha 4/CD49d Antibody (Natalizumab) - Chimeric - Azide and BSA Free

Flow Cytometry: Integrin alpha 4/CD49d Antibody (Natalizumab) - Chimeric [NBP2-75917] - Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of Natalizumab (NBP2-75917, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: Flow, B/N
• Clone: Natalizumab
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Integrin alpha 4/CD49d Antibody (Natalizumab) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: The RAMOS cell line, a non-EBV releasing cell line, injected into mice.
• Specificity: This antibody binds to the alpha 4 subunit of (alpha)4(beta)1 (also known as very late antigen 4 (VLA-4) or CD49d-CD29) and (alpha)4(beta)7 integrin
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGA4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
• Application Notes: NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original human IgG4 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha 4/CD49d Antibody (Natalizumab) - Chimeric - Azide and BSA Free

• 269C wild type
• biosimilar antibody
• biosimilars
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• ITGA4
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha
• VLA-4

Background

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA4