Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free

Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.

Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (NBP2-52680, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (NBP2-52680) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52680, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free [NBP2-52680] - Abundance of two potential Hla receptors & of caveolin-1 as a potential pore-stabilizing factor.A: Representative examples of immune fluorescence assays using epifluorescence microscopy (nuclei counterstained using DAPI) that were performed on 16HBE14o-, S9 or A549 cells grown on coverslips using antibodies against ADAM10, the alpha 5 beta 1 integrin or against caveolin-1. Staining appearing in red represents specific labelling of the respective proteins. Scale bars: 10 μm. B: Semi-quantitative determination of primary & secondary antibody-mediated fluorescence in suspended individual cells by flow cytometry. During flow cytometry, the fluorescence of the antibody-tagged proteins per cell was measured & the respective medians of the detected peaks were used for calculating the means ± S.D. for the biological replicates (n = 4, each). Individual means were tested for significant differences using Student’s t-test or Welch’s t-test: * = p ≤ 0.05, ** = p ≤ 0.01 or *** = p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32470006), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC, B/N, CyTOF-ready
• Clone: M200 (Volociximab)
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Heterodimeric alpha 5 Beta 1 Fc fusion protein
• Specificity: The antibody binds to human (alpha)5(beta)1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGA5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
• Application Notes: This antibody is Cytof ready. NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques. Use in ICC/IF was reported in scientific literature (PMID: 30611716). The antibody binds specifically to Integrin alpha 5 beta 1, which is part of the superfamily of glycoprotein transmembrane receptors for ligands such as fibronectin, vitronectin, laminins and collagens. The Integrin alpha 5 beta 1 receptor plays a key role in cellular processes such as inflammation, cell proliferation, angiogenesis and tumour metastasis. Comprising the variable domain of the original mouse antibody, a chimeric mouse:human IgG4 version of this antibody has been tested in vivo: In cynomolgus monkeys with laser-induced choroidal neovascularization (CNV), in rabbit models and in human volunteers. The antibody reached Phase II clinical trials with PDL Biopharma and Biogen-Idec. Use in IHC reported in secitific publication PMID: 32449990. Integrin alpha 5 beta 1 Antibody validated for Immunohistochemstry-Paraffin from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free

• biosimilar antibody
• biosimilars
• CD49 antigen-like family member E
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha polypeptide
• fibronectin receptor, alpha subunit
• FNRA
• Integrin alpha 5 beta 1
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5/SLC9B1 fusion
• very late activation protein 5, alpha subunit
• VLA-5
• VLA5A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 5 beta 1 Antibody (NBP2-52680)(6)

Publications using NBP2-52680

• Phillips AT, Boumil EF, Castro N Et al. USP10 Promotes Fibronectin Recycling, Secretion, and Organization Investigative ophthalmology & visual science 2021-10-04 [PMID: 34665194] (Human)
• Ludwig BS, Tomassi S, Di Maro S et al. Pharmacological Inhibition of Brain EGFR Activation By a BBB-penetrating Inhibitor, AZD3759, Attenuates alpha-synuclein Pathology in a Mouse Model of alpha-Synuclein PropagationThe organometallic ferrocene exhibits amplified anti-tumor activity by targete Biomaterials 2021-03-12 [PMID: 33756215] (ICC/IF, Human)
• Moller N, Ziesemer S et al. S. aureus alpha-toxin monomer binding and heptamer formation in host cell membranes - Do they determine sensitivity of airway epithelial cells toward the toxin? PLoS One 2020-05-30 [PMID: 32470006] (FLOW, Human)
• Smith PC, Metz C, de la PeNa A et al. Galectin-8 mediates fibrogenesis induced by cyclosporine in human gingival fibroblasts J. Periodont. Res. 2020-05-25 [PMID: 32449990] (IHC, Human)
• Li L, Hu X, Eid JE et al. Mutant IDH1 Depletion Downregulates Integrins and Impairs Chondrosarcoma Growth Cancers (Basel) 2020-01-06 [PMID: 31935911] (Human)
• Laurenzana A, Margheri F, Biagioni A, et al EGFR/uPAR interaction as druggable target to overcome vemurafenib acquired resistance in melanoma cells EBioMedicine. 2019-01-02 [PMID: 30611716] (ICC/IF, Human)

Review for Integrin alpha 5 beta 1 Antibody (NBP2-52680) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Gail Seigel IHC-P Human 10/31/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Xenograft Tumor,NCI-N87 human gastric carcinoma cell line
• Species: Human
• Lot: T2143C05

Comments

• Comments: Paraffin sections were rehydrated through xylene and alcohols, boiled 25 minuted in sodium citrate antigen retrieval buffer and immunostained using the CRF Anti-Polyvalent HRP Polymer kit (ScyTek, Inc. Logan, Utah). ITGA5 (Novus) was used as the primary antibody (1:200 dilution) and incubated for 1 h. Negative controls received non-specific rabbit antibody instead of primary antibody. HRP Polymer and diaminobenzidine (DAB) were used according to the CRF kit to develop brown reaction product. After DAB staining and a water rinse, paraffin sections were counterstained with hematoxylin for 1 min, rinsed in water, then 15s in acid ethanol, a water rinse, followed by dehydration through a series of graded alcohols to xylene. Paraffin slides were mounted in Permount (Fisher Scientific, Pittsburgh, PA). Digital images were captured with a SONY ICX 285AL SPOT camera (Diagnostic Instruments, Sterling Heights, MI).

Bioinformatics

• Gene Symbol: ITGA5