Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free

Flow Cytometry: Rat Monoclonal Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) [NBP3-09130] - Separation of murine bone marrow cells stained using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 ug/ml, DAR APC) from murine bone marrow cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining).

Flow Cytometry: Rat Monoclonal Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - Flow cytometry surface staining pattern of murine bone marrow using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 μg/ml, DAR APC).

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC
• Clone: 5H10-27 (MFR5)
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free Summary

• Immunogen: C57BL/6 x A/J)F1 murine mast cell line
• Specificity: The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free

• CD49 antigen-like family member E
• CD49e antigen
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha subunit
• FNRAVLA5A
• Integrin alpha 5
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5
• very late activation protein 5, alpha subunit
• VLA-5 alpha
• VLA-5

Background

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA5