Integrin alpha 5/CD49e Antibody (SNAKA51) - BSA Free

• Reactivity: Hu, Mu
• Applications: WB, Func, ICC/IF
• Clone: SNAKA51
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha 5/CD49e Antibody (SNAKA51) - BSA Free Summary

• Immunogen: Whole cells corresponding to human Integrin alpha 5/CD49e
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Functional
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Functional: A representative lot of this antibody converts Integrin alpha5 beta 1 into a ligand competant form and promotes fibronectin binding. Immunocytochemistry/Immunofluorescence: A representative lot of this antibody detected Integin alpha 5 in Primary human foreskin fibroblasts (hFF). Positive control(s): HeLa cells.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30194266).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha 5/CD49e Antibody (SNAKA51) - BSA Free

• CD49 antigen-like family member E
• CD49e antigen
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha subunit
• FNRAVLA5A
• Integrin alpha 5
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5
• very late activation protein 5, alpha subunit
• VLA-5 alpha
• VLA-5

Background

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 5/CD49e Antibody (NBP2-50146)(3)

Publications using NBP2-50146

• Kurisinkal EE, Caroprese V, Koga MM et al. Selective Integrin alpha5 beta1 Targeting through Spatially Constrained Multivalent DNA-Based Nanoparticles Molecules (Basel, Switzerland) 2022-08-04 [PMID: 35956918] (ICC/IF, Human)
• Sundararaman A, Fukushima Y, Norman JC et al. RhoJ Regulates alpha 5 beta 1 Integrin Trafficking to Control Fibronectin Remodeling during Angiogenesis Curr. Biol. 2020-01-01 [PMID: 32302585]
• Albarran-Juarez J, Iring A, Wang S et al. Piezo1 and Gq/G11 promote endothelial inflammation depending on flow pattern and integrin activation. J. Exp. Med. 2018-09-07 [PMID: 30194266] (Mouse)

Bioinformatics

• Gene Symbol: ITGA5