Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Separation of human CD103 positive cells (red-filled) from CD103 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on human peripheral blood cells with anti-CD103 (Ber-ACT8) APC.

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) FITC.

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) PE.

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).

• Reactivity: Hu, Pm
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clone: Ber-ACT8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free Summary

• Additional Information: Clone Ber-ACT8 was used by HLDA to establish CD designation.
• Immunogen: HTLV-1 induced human T cell line MAPS16
• Specificity: The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes. HLDA V; WS Code A067
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

• antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide
• CD103 antigen
• CD103
• HML-1 antigen
• HUMINAE
• Integrin alpha E
• integrin alpha-E
• Integrin alpha-IEL
• integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide)
• ITGAE
• MGC141996
• Mucosal lymphocyte 1 antigen

Background

CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha E/CD103 Antibody (NBP1-97564)(2)

Publications using NBP1-97564

• KrAmer B, Nalin A, Ma F et al. Single-cell RNA sequencing identifies a population of human liver-type ILC1s Cell Reports 2023-01-01 [PMID: 36640314]
• Hezaveh K, Shinde RS, KlOtgen A et al. Tryptophan-derived microbial metabolites activate the aryl hydrocarbon receptor in tumor-associated macrophages to suppress anti-tumor immunity Immunity 2022-02-08 [PMID: 35139353]

Bioinformatics

• Gene Symbol: ITGAE