Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free

Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) [NBP2-52708] - Flow-cytometry using anti-CD29 antibody K20 (NBP2-52708) Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of K20 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: K20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody. Clone K20 was used by HLDA to establish CD designation.
• Immunogen: Human T-cell lymphoma cells injected into Biozzi high-responder mice.
• Specificity: Recognises human CD29.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (NBP2-52708)(2)

Publications using NBP2-52708

• Miao MZ, Su QP, Cui Y et al. Redox-active endosomes mediate ?5?1 integrin signaling and promote chondrocyte matrix metalloproteinase production in osteoarthritis Science signaling 2023-10-31 [PMID: 37906629]
• Quaglia F, Krishn SR, Sossey-Alaoui K et al. The NOGO receptor NgR2, a novel alpha V beta 3 integrin effector, induces neuroendocrine differentiation in prostate cancer Scientific reports 2022-11-07 [PMID: 36344556] (IP, Human)

FAQs for Integrin beta 1/CD29 Antibody (NBP2-52708). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1