Integrin beta 1/CD29 Antibody (TS2/16) [FITC]

Product Discontinued

Integrin beta 1/CD29 Antibody (TS2/16) [FITC] Summary

• Immunogen: The immunogen was the full length version of the protein: CD29 (TS2/16)
• Marker: Stem Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry
  • Immunoprecipitation
• Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections, and activation of integrin beta 1. Immunohistochemistry-Frozen and Immunoprecipitation were reported in scientific literature.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 7698991). Expected cross reactivity based on sequence identity: Bovine

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Integrin beta 1/CD29 Antibody (TS2/16) [FITC]

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

The TS2/16 monoclonal antibody reacts with human CD29, also known as integrin beta 1, an approximately 130 kDa single-pass transmembrane glycoprotein. CD29 complexes with one of nine integrin alpha subunits to form the very late antigen (VLA) subfamily of adhesion molecules. Integrin heterodimers containing CD29 are involved in cell-cell and cell-matrix adhesion. CD29 is expressed broadly on lymphocytes and monocytes, with lower levels of expression on granulocytes. The TS2/16 antibody has been found to possess activating activity for beta 1 integrins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (NBP1-99998)(3)

Publications using NBP1-99998

• Weber C, Alon R, Moser B, Springer TA. Sequential regulation of alpha4beta1 and alpha5beta1 integrin avidity by CC chemokines in monocytes: implications for transendothelial chemotaxis. J Cell Biol. [PMID: 8769427] (Func)
  
  Details:
  Citation using the FITC form of this antibody.
• Sanchez-Madrid F, Krensky AM, Ware CF et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3 Proc Natl Acad Sci USA [PMID: 6984191] (IP, Func)
  
  Details:
  Used the FITC form of this antibody.
• Hemler ME et al. J. Immunol. 132:3011. 1984-01-01 [PMID: 6327814] (IF/IHC, IP)

FAQs for Integrin beta 1/CD29 Antibody (NBP1-99998). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1