Integrin beta 4/CD104 Antibody (10B10D5)

Western Blot: Integrin beta 4/CD104 Antibody (10B10D5) [NBP2-37392] - Analysis using ITGB4 mouse mAb against A549 (1), A431 (2), MCF-7 (3) and SW620 (4) cell lysate.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (10B10D5) [NBP2-37392] - Analysis of esophageal cancer tissues using ITGB4 mouse mAb with DAB staining.

Flow Cytometry: Integrin beta 4/CD104 Antibody (10B10D5) [NBP2-37392] - Analysis of A549 cells using ITGB4 mouse mAb (green) and negative control (purple).

Western Blot: Integrin beta 4/CD104 Antibody (10B10D5) [NBP2-37392] - Analysis using ITGB4 mAb against human ITGB4 recombinant protein. (Expected MW is 24 kDa)

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (10B10D5) [NBP2-37392] - Analysis of cervical cancer tissues using ITGB4 mouse mAb with DAB staining.

ELISA: Integrin beta 4/CD104 Antibody (10B10D5) [NBP2-37392] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 10B10D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Integrin beta 4/CD104 Antibody (10B10D5) Summary

• Immunogen: Purified recombinant fragment of human Integrin beta 4/CD104 (AA: 1619-1822) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 202 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Integrin beta 4/CD104 Antibody (10B10D5)

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 4/CD104 Antibody (NBP2-37392)(2)

Publications using NBP2-37392

• Hara T, Kasagi Y, Wang J et al. CD73+ epithelial progenitor cells which contribute to homeostasis and renewal are depleted in eosinophilic esophagitis Cellular and molecular gastroenterology and hepatology 2022-01-30 [PMID: 35108658] (IF/IHC, Human)
• Zang D, Zhang C, Li C et al. LPPR4 promotes peritoneal metastasis via Sp1/integrin alpha/FAK signaling in gastric cancer Am J Cancer Res 2020-03-01 [PMID: 32266108] (Human)

Bioinformatics

• Gene Symbol: ITGB4