IRP2 Antibody

Western Blot: IRP2 Antibody [NB100-1797] - Detection of IRP2 in mouse liver lysate.

Immunocytochemistry/Immunofluorescence: IRP2 Antibody [NB100-1797] - IRP2 antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Discontinued

IRP2 Antibody Summary

• Immunogen: A synthetic peptide made to an N-terminal portion of the human IRP2 protein sequence (between residues 1-100). [UniProt P48200]
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IREB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:500
• Application Notes: This IRP2 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In ICC/IF, cytoplasmic staining was observed in NIH-3T3 cells.
• Control:

  NIH 3T3 Whole Cell Lysate

Reactivity Notes

Mouse. Human reactivity reported in scientific literature (PMID: 21865595)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IRP2 Antibody

• ACO3
• EC 4.2.1.3
• FLJ23381
• IRE-BP 2
• Iron regulatory protein 2
• iron-responsive element binding protein 2
• iron-responsive element-binding protein 2
• IRP2IRP2AD

Background

Iron regulatory proteins -1 and -2 (IRP1, IRP2) are homologous cytoplasmic RNA-binding proteins that controls cellular iron metabolism and maintain systemic iron homeostasis in vertebrates along with serving some additional iron-independent functions. IRP2 belongs to aconitase/IPM isomerase family and it binds to IRES (iron-responsive elements), which are stem-loop structures found in 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IRP2-IRE binding results in the repression of its mRNA translation and its binding to transferrin receptor mRNA inhibits the degradation of this otherwise quickly degradable mRNA. IRP2 is a substrate of FBXL5, an F-box protein that assembles together with Skp1, Cul1 and Rbx1 into an E3 ubiquitin ligase complex. When iron/O2 are high, IRP2 gets ubiquitinated/degraded by SCF complex containing FBXL5, whereas under conditions of low iron/O2, FBXL5 is degraded preventing IRP2 ubiquitination leading to its RNA-binding activity. IRP2 deficient mice depicts microcytosis, misregulated iron metabolism (in CNS, intestine as well as liver associated with FTH1 and FTL up-regulation) and iron deficiency in splenic macrophages associated with down-regulation of FTH1, FTL, and FPN1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRP2 Antibody (NB100-1797)(2)

Publications using NB100-1797

• Maeda M, Hasegawa H, Hyodo T et al. ARHGAP18, a GTPase-activating protein for RhoA, controls cell shape, spreading, and motility. Mol Biol Cell 2011-10-01 [PMID: 21865595] (Human)
• Song D, Lee FS. Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. J Biol Chem 2011-05-06 [PMID: 21367862] (WB, Mouse)

Bioinformatics

• Gene Symbol: IREB2
• Entrez:
  • 3658(Human)
  • 64602(Mouse)
• Uniprot:
  • Q811J3(Mouse)