![Western Blot: Jade-1/PHF17 Antibody [NBP1-47280] - Detection of human and mouse Jade-1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Jade-1 antibody NBP1-47280 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Jade-1 was also immunoprecipitated by another rabbit anti-Jade-1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Jade-1-PHF17-Antibody-Western-Blot-NBP1-47280-img0003.jpg "Western Blot: Jade-1/PHF17 Antibody [NBP1-47280] - Detection of human and mouse Jade-1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Jade-1 antibody NBP1-47280 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Jade-1 was also immunoprecipitated by another rabbit anti-Jade-1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
| Immunogen | The immunogen for this product maps to a region between residue 650 and 700 of human Gene for Apoptosis and Differentiation in Epithelia using the numbering given in entry NP_955352.1 (GeneID 79960). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | JADE1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE. |
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| Theoretical MW | 96 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-47280 | Applications | Species |
|---|---|---|
| Wan G, Hu X, Liu Y et al. A novel non-coding RNA lncRNA-JADE connects DNA damage signalling to histone H4 acetylation. EMBO J. 2013-10-30 [PMID: 24097061] (WB, Human, Mouse) | WB | Human, Mouse |
Secondary Antibodies |
Isotype Controls |
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