Jarid1A Antibody (JB36-60)

Western Blot: Jarid1A Antibody (JB36-60) [NBP2-75548] - Analysis of KDM5A on A549 cell using anti-KDM5A antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Jarid1A Antibody (JB36-60) [NBP2-75548] - Staining KDM5A in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: Jarid1A Antibody (JB36-60) [NBP2-75548] - Analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Jarid1A Antibody (JB36-60) [NBP2-75548] - Staining KDM5A in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Jarid1A Antibody (JB36-60) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Jarid1A aa 1,360-1,690 / 1,690. (SwissProt: P29375 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KDM5A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:400
  • Western Blot 1:500
• Theoretical MW: 192 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Jarid1A Antibody (JB36-60)

• EC 1.14.11
• EC 1.14.11.-
• EC 1.14.11.67
• Histone demethylase JARID1A
• Jarid1A
• JARID1AJumonji, AT rich interactive domain 1A (RBP2-like)
• Jumonji, AT rich interactive domain 1A (RBBP2-like)
• Jumonji/ARID domain-containing protein 1A
• KDM5A
• lysine (K)-specific demethylase 5A
• RBBP2
• RBBP-2
• RBBP2lysine-specific demethylase 5A
• RBP2
• RBP2retinoblastoma binding protein 2
• Retinoblastoma-binding protein 2jumonji, AT rich interactive domain 1A

Background

Jarid1A is encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDM5A