KAP1 Antibody (4E6) - Azide and BSA Free

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6. Analysis of TRIM28 expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi ( Cat # H00010155-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody (M01) clone 4E6 (Cat # H00010155-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6. Analysis of TRIM28 expression in PC-12.

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6 Analysis of TRIM28 expression in Hela S3 NE.

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M01), clone 4E6.Lane 1: TRIM28 transfected lysate(88.5 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, KD
• Clone: 4E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

KAP1 Antibody (4E6) - Azide and BSA Free Summary

• Immunogen: TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIER
• Specificity: TRIM28 (4E6)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM28
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation, and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KAP1 Antibody (4E6) - Azide and BSA Free

• E3 SUMO-protein ligase TRIM28
• EC 6.3.2.-
• FLJ29029
• KAP1
• KAP-1
• KAP1KRAB-associated protein 1
• KRIP-1
• Nuclear corepressor KAP-1
• RNF96
• RNF96KRAB-interacting protein 1
• TF1B
• TIF1B
• TIF1-beta
• TIF1BRING finger protein 96
• transcription intermediary factor 1-beta
• transcriptional intermediary factor 1-beta
• TRIM28
• tripartite motif containing 28
• tripartite motif-containing 28
• Tripartite motif-containing protein 28

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KAP1 Antibody (H00010155-M01)(1)

Publication using H00010155-M01

• Dihazi H, Dihazi GH, Jahn O et al. Multipotent Adult Germline Stem Cells and Embryonic Stem Cells Functional Proteomics Revealed an Important Role of Eukaryotic Initiation Factor 5A (Eif5a) in Stem Cell Differentiation. J Proteome Res. 2011-02-24 [PMID: 21344951]

Bioinformatics

• Gene Symbol: TRIM28
• Entrez:
  • 10155(Human)
• OMIM:
  • 601742(Human)
• Uniprot:
  • AAH04978(Human)