Ku80/XRCC5 Antibody (3D8) - Azide and BSA Free

Western Blot: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - XRCC5 monoclonal antibody (M02), clone 3D8. Analysis of XRCC5 expression in A-431.

Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of monoclonal antibody to XRCC5 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of monoclonal antibody to XRCC5 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.

Western Blot: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of XRCC5 expression in transfected 293T cell line by XRCC5 monoclonal antibody (M02), clone 3D8.Lane 1: XRCC5 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Detection limit for recombinant GST tagged XRCC5 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, NULL
• Clone: 3D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Ku80/XRCC5 Antibody (3D8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: XRCC5 (AAH19027.1, 1 a.a. ~ 732 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQRQVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQPGSQQADSLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKCDISLQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMISLEGEDGLDEIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQERVKKTWTVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQGFRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQLNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPIQQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGIALITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI
• Localization: Nuclear
• Specificity: XRCC5 - X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kDa)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: XRCC5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Quantification
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ku80/XRCC5 Antibody (3D8) - Azide and BSA Free

• 80kDa
• ATP-dependent DNA helicase 2 subunit 2
• ATP-dependent DNA helicase II 80 kDa subunit
• CTC85
• CTCBF
• EC 3.6.4.-
• FLJ39089
• G22P2
• KARP1
• KARP-1
• Ku80
• Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen
• Ku86
• Ku86DNA repair protein XRCC5
• KUB2
• NFIV
• Thyroid-lupus autoantigen
• TLAA
• X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit
• X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD)
• X-ray repair cross-complementing protein 5
• X-ray repair, complementing defective, repair in Chinese hamster
• XRCC5

Background

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ku80/XRCC5 Antibody (H00007520-M02)(4)

Publications using H00007520-M02

• Hasegawa T, Someya M, Hori M et al. Expression of Ku70 predicts results of radiotherapy in prostate cancer. Strahlenther Onkol 2016-07-27 [PMID: 27465041]
• Hori M, Someya M, Matsumoto Y et al. Influence of XRCC4 expression in esophageal cancer cells on the response to radiotherapy. Med Mol Morphol 2016-06-23 [PMID: 27338590]
• Nomura F, Sogawa K, Noda K et al. Serum anti-Ku86 is a potential biomarker for early detection of hepatitis C virus-related hepatocellular carcinoma. Biochem Biophys Res Commun. 2012-04-19 [PMID: 22554520]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: XRCC5
• Entrez:
  • 7520(Human)
• OMIM:
  • 194364(Human)
• Uniprot:
  • AAH19027(Human)