L1CAM Antibody (555) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse L1CAM Monoclonal Antibody (Catalog # MAB5674, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0105B).

Neuro-2A cells were stained with Rat Anti-Mouse L1CAM Monoclonal Antibody (Catalog # MAB5674, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 555
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

L1CAM Antibody (555) [Unconjugated] Summary

• Immunogen: Mouse cerebellum-derived partially purified L1CAM
• Specificity: Detects mouse L1CAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM, rhNCAM-L1, rhOBCAM, recombinant mouse (rm)MAdCAM-1, or rmOCAM is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: L1CAM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for L1CAM Antibody (555) [Unconjugated]

• antigen identified by monoclonal R1
• CAML1
• CAML1N-CAML1
• CD171 antigen
• CD171
• HSAS
• HSAS1
• L1 cell adhesion molecule
• L1CAM
• MASA
• MIC5
• N-CAM-L1
• NCAM-L1
• neural cell adhesion molecule L1
• S10
• SPG1

Background

L1CAM, also known as Neural Cell Adhesion Molecule L1 (NCAM-L1) and CD171, is a 200‑220 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily and L1/neurofascin/NgCAM family (1, 2). L1CAM is expressed by neurons, especially by growing axons on their growth cones (2). Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express L1CAM (2). Mature mouse L1CAM consists of a 1104 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains and 5 fibronectin type-III domains, a 23 aa transmembrane region, and a 114 aa cytoplasmic tail (3). Mouse L1CAM shares 88% aa sequence identity with human L1CAM. L1CAM is critical for neural development. Specifically, L1CAM plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination (4). L1CAM mediates homophilic cell-cell interaction but also binds heterophilically with Axonin-1, CD24, CD9, Neurocan and several Integrins (4). L1CAM can undergo membrane‑proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble ECD and the generation of a membrane‑bound stub (4). The soluble ECD can serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration (4). L1CAM also plays a role in the ontogeny of human tumors, and its expression is linked to poor prognosis (1). Overexpression promotes tumor cell invasion and motility, growth in nude mice, and tumor metastasis (1). Proteolytic processing by ADAM10 and
presenilin/ gamma -secretase is essential for the nuclear signaling of L1CAM in human carcinoma cell lines (1). Defects in L1CAM are the cause of the neurological MASA/CRASH syndrome (5, 6).

1. Riedle, S. et al. (2009) Biochem. J. 420:391.
2. Kenwrick, S. and P. Doherty (1998) Bioessays 20:668.
3. Crossin, K.L. and L.A. Krushel (2000) Dev. Dyn. 218:260.
4. Maretzky, T. et al. (2005) Mol. Cell. Biol. 25:9040.
5. Kamiguchi, H. et al. (1998) Mol. Cell Neurosci. 12:48.
6. Striha, L. et al. (2000) J. Child Neurol. 15:239.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for L1CAM Antibody (MAB5674)(7)

Publications using MAB5674

• Ryosuke Tsuchimochi, Keitaro Yamagami, Naoko Kubo, Naoya Amimoto, Fabian Raudzus, Bumpei Samata et al. Viral delivery of L1CAM promotes axonal extensions by embryonic cerebral grafts in mouse brain Stem Cell Reports 2023-04-11 [PMID: 36963389]
• R Zhou, B Han, R Nowak, Y Lu, E Heller, C Xia, AH Chishti, VM Fowler, X Zhuang Proteomic and functional analyses of the periodic membrane skeleton in neurons Nature Communications, 2022-06-09;13(1):3196. 2022-06-09 [PMID: 35680881] (ICC, Mouse)
• A Sobiepanek, M Milner-Kra, P Musolf, T Starecki, T Kobiela Anandamide-Modulated Changes in Metabolism, Glycosylation Profile and Migration of Metastatic Melanoma Cells Cancers, 2022-03-10;14(6):. 2022-03-10 [PMID: 35326572] (ELISA Capture, Mouse)
• B Samata, R Takaichi, Y Ishii, K Fukushima, H Nakagawa, Y Ono, J Takahashi L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain Front Cell Neurosci, 2020-02-19;14(0):31. 2020-02-19 [PMID: 32140099] (IHC, Mouse)
• Elizabeth A. Swanson, Jonathan W. Nelson, Sophia Jeng, Kayla J. Erspamer, Chao-Ling Yang, Shannon McWeeney et al. Salt-sensitive transcriptome of isolated kidney distal tubule cells Physiological Genomics 2019-04-01 [PMID: 30875275]
• Yijian Li, Shujia Huo, Yajie Fang, Ting Zou, Xianliang Gu, Qin Tao et al. ROCK Inhibitor Y27632 Induced Morphological Shift and Enhanced Neurite Outgrowth-Promoting Property of Olfactory Ensheathing Cells via YAP-Dependent Up-Regulation of L1-CAM Frontiers in Cellular Neuroscience 2018-12-11 [PMID: 30618636]
• He , Xiaowen, Knepper , Michael, Ding , Cheng, Li , Jun, Castro , Suita, Siddiqui , Maham, Schachner , Melitta Promotion of spinal cord regeneration by neural stem cell-secreted trimerized cell adhesion molecule l1. PLoS ONE, 2012-09-25;7(9):e46223. 2012-09-25 [PMID: 23049984] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: L1CAM