Lactase-like Protein/LCTL Antibody

Western blot shows lysates of A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Lactase-like Protein/LCTL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5984) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lactase-like Protein/LCTL at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lactase-like Protein/LCTL Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Lactase-like Protein/LCTL
  Arg23-Glu541
  Accession # Q6UWM7
• Specificity: Detects human Lactase-like Protein/LCTL in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse Lactase-like Protein/LCTL is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LCTL
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lactase-like Protein/LCTL Antibody

• FLJ33279
• KLG
• klotho gamma
• Klotho/lactase-phlorizin hydrolase-related protein
• KLPH
• KLPHKL lactase phlorizin hydrolase
• Lactase like Protein
• Lactase-like Protein
• lactase-like
• LCTL

Background

LCTL (also Lactase-like Protein and Klotho/lactase-phlorizin hydrolase-related protein/KLPH) is a 62 kDa (predicted) member of the glycosyl hydrolase 1 family of proteins. It is expressed in skin and kidney, and may participate in endocrine FGF19 subfamily signaling pathways. Mature human LCTL is a 546 amino acid (aa) type I transmembrane protein that appears to be present in the ER. It contains an apparently nonfunctional glycosidase domain (aa 33‑503), plus a very short five aa cytoplasmic segment that may function as an ER-retention signal. There is one potential alternative start site at Met174. Over aa 23‑541, human LCTL shares 86% aa identity with mouse LCTL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lactase-like Protein/LCTL Antibody (AF5984)(1)

Publication using AF5984

• Ji-Hee Kim, Eun Young Park, Kyu-Hee Hwang, Kyu-Sang Park, Seong Jin Choi, Seung-Kuy Cha Soluble alpha Klotho downregulates Orai1-mediated store-operated Ca2+ entry via PI3K-dependent signaling Pflügers Archiv - European Journal of Physiology 2021-04-01 [PMID: 33386992]

Bioinformatics

• Gene Symbol: LCTL
• Uniprot:
  • Q6UWM7(Human)