Goat anti-Human Lambda Light Chain Secondary Antibody [HRP]

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Concentration: 1.0 mg/ml

Goat anti-Human Lambda Light Chain Secondary Antibody [HRP] Summary

• Immunogen: Human Lambda Light Chain
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with lambda light chains common to all human immunoglobulins. No antibody was detected against kappa light chain or non-immunoglobulin serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGLC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:2000-1:20000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS) containing 0.2%BSA
• Preservative: 0.05% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Goat anti-Human Lambda Light Chain Secondary Antibody [HRP]

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain are present in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lambda Light Chain Antibody (NB7471)(1)

Publication using NB7471

• Pink D, Luhrs KA, Zhou L et al. High efficacy vasopermeability drug candidates identified by screening in an ex ovo chorioallantoic membrane model. Sci Rep. [PMID: 26510887]
  
  Details:
  Citation using the HRP form of this antibody.

Bioinformatics

• Gene Symbol: IGLC1