Laminin alpha 5 Antibody (CL3118) - BSA Free

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining in human placenta and lymph node tissues. Corresponding LAMA5 RNA-seq data are presented for the same tissues.

Western Blot: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Altered ECM protein gene expression in liver tissue during aging. Immunofluorescence analysis of liver tissue sections from 2-month-old and 22-month-old rats (n = 3) with antibodies against (d, e) LAMA5 (Laminin alpha 5). Cell nuclei were stained with DAPI (blue; scale bars: 20 um). Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.13131), licensed under a CC-BY license.

Western Blot: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Analysis of purified human recombinant Laminin-511, Laminin-521, Laminin-121, Laminin-221, Laminin-332 and Laminin-411.

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining of human kidney shows strong membranous positivity in glomeruli, as well as in basement membrane of renal tubules.

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining of human placenta shows moderate positivity in basement membrane of trophoblastic cells.

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining of human colon shows moderate positivity in basement membrane of glandular cells.

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining of human lymph node shows no positivity in lymphoid cells as expected.

Immunocytochemistry/ Immunofluorescence: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Altered ECM protein gene expression in liver tissue during aging. (a) Hierarchical cluster analysis of differentially expressed genes in whole liver tissue from 2‐month‐old & 22‐month‐old rats. (b) Scatter plot of gene expression of whole liver tissue from both age groups (n = 3 per age group, fold change > 2, p < .05, ANOVA). (c) Expression analyses of genes encoding for ECM proteins by qPCR. Mean expression of liver tissue samples from young rats was set to 100% (n = 8–10 for 2‐month‐old & n = 8–9 for 22‐month‐old rats, *p < .05). Immunofluorescence analysis of liver tissue sections from 2‐month‐old & 22‐month‐old rats (n = 3) with antibodies against (d, e) LAMA5, (f, g) COL1, & (h, i) COL4 (red). Cell nuclei were stained with DAPI (blue; scale bars: 20 µm). (j) Proteome analysis of decellularized rat liver tissue. Mean intensities of samples from young rats were set to 100% (n = 3, *p < .05). (c, j) Data are presented as means ± SEM Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157808), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Rt
• Applications: WB, IHC, KD
• Clone: CL3118
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from O15230, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMA5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32157808).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Laminin alpha 5 Antibody (CL3118) - BSA Free

• KIAA0533
• KIAA1907
• laminin alpha-5 chain
• laminin subunit alpha-5
• laminin, alpha 5
• Laminin-10 subunit alpha
• Laminin-11 subunit alpha
• Laminin-15 subunit alpha

Background

Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin alpha 5 Antibody (NBP2-42391)(5)

Publications using NBP2-42391

• Martha Lilia T, Laura Chavez M, Erick G et al. Expression of Collagen VI, Anticollagenase, Laminin, MM9, Claudins 1 and 5, N and E Cadherins in Choroid Plexus Tumors Archives of Pathology and Clinical Research 2023-10-25
• Altera A, Tosi GM, Regoli M et al. The extracellular matrix complexity of idiopathic epiretinal membranes and the bilaminar arrangement of the associated internal limiting membrane in the posterior retina Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2021-03-24 [PMID: 33760980]
• Rohn F, Kordes C, Buschmann T et Al. Impaired integrin alplha 5 /beta 1 -mediated hepatocyte growth factor release by stellate cells of the aged liver Aging Cell 2020-03-11 [PMID: 32157808] (IHC-F, IF/IHC, Rat)
• Liu P, Chen H, Yan L, Sun Y Laminin alpha 5 modulates fibroblast proliferation in epidural fibrosis through the PI3K/AKT/mTOR signaling pathway Mol Med Report 2020-01-28 [PMID: 32016453] (IHC-P)
• Gordon-Weeks A, Lim SY, Yuzhalin A et al. Tumour-Derived Laminin alpha 5 (LAMA5) Promotes Colorectal Liver Metastasis Growth, Branching Angiogenesis and Notch Pathway Inhibition Cancers (Basel) 2019-05-06 [PMID: 31064120] (WB, Human)

Bioinformatics

• Gene Symbol: LAMA5
• Uniprot:
  • O15230(Human)