LAP1 Antibody (RL13) - BSA Free

Immunocytochemistry/Immunofluorescence: LAP1 Antibody (RL13) [NB120-2737] - Analysis of LAP1 using anti-LAP1 monoclonal antibody shows staining in NS-1 Cells.

Immunohistochemistry-Paraffin: LAP1 Antibody (RL13) [NB120-2737] - Immunohistochemistry was performed on rat lymph node tissue. To expose target protein, antigen was retreived. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry: LAP1 Antibody (RL13) [NB120-2737] - Immunohistochemistry was performed on rat breast tissue. To expose target protein, antigen was retreived. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:50 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry-Paraffin: LAP1 Antibody (RL13) [NB120-2737] - Immunohistochemistry was performed on rat colon tissue. To expose target protein, antigen was retreived. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LAP1 mouse monoclonal antibody at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: RL13
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LAP1 Antibody (RL13) - BSA Free Summary

• Immunogen: Pore complex-lamina fraction isolated from rat liver nuclear envelopes.
• Localization: Immunofluorescence staining of LAP 1 in rat liver with this antibody results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.
• Specificity: Detects lamin associated polypeptide (LAP) 1A, 1B & 1C from rat tissues. This does not cross-react with LAP 2.This detects 3 distinct proteins of molecular weights 75, 68 and 55 kDa representing LAP 1A, 1B and 1C, respectively in rat liver nuclear envelope fractions. Immunofluorescence staining of LAP 1 in rat liver with NB120-2737 results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOR1AIP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:200

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for LAP1 Antibody (RL13) - BSA Free

• lamina-associated polypeptide 1B
• torsin A interacting protein 1

Background

In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B & 1C share a high degree of sequence homology while LAP 2 is distinct from the others.LAP 1A & B bind lamins A, C and B1, while LAP 2 binds lamin B1 only.In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOR1AIP1
• Entrez:
  • 117284(Rat)