LDH-Cytotoxicity Assay Kit (Colorimetric)

Product Discontinued

LDH-Cytotoxicity Assay Kit (Colorimetric) Summary

• Description: Quantitate cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Rapid, sensitive, convenient colorimetric assay.
  
  Detection method: Absorbance at 500 nmApplications: Convenient and non-radioactive alternative to the [3H]-thymidine and the [51Cr] release assays.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65391)
• Kit Type: Assay Kit (Colorimetric)

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. The LDH-Cytotoxicity Assay Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or are unstable, LDH is a stable cytoplasmic enzyme present in all cells and is rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.