LDH-Cytotoxicity Assay Kit (Fluorometric)

Product Discontinued

LDH-Cytotoxicity Assay Kit (Fluorometric) Summary

• Description: Rapid, highly sensitive and high-throughput adaptable assay to quantitate cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Detects as few as 100 cells.
  
  Detection method: Fluorescence (Ex/Em = 535/587 nm) Applications: Measurement of released LDH in culture medium. Evaluation of cytotoxicity effects of test compounds.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab197004)
• Kit Type: Assay Kit (Fluorometric)

Applications/Dilutions

Publications

Read Publication using NBP2-54851.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

Lactate Dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. This Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for LDH-Cytotoxicity Kit (NBP2-54851)(1)

Publication using NBP2-54851

• Jan A, Jansonius B, Delaidelli A et al. Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity. Acta Neuropathol Commun. 2018-07-02 [PMID: 29961428] (Human)