Human Leptin R ELISA Kit (Colorimetric)

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ELISA: Leptin R ELISA Kit [HRP] [KA0027] - Standard curve

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA0027
    • Availability
      Product Discontinued

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Human Leptin R ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard curve. LEPR (Human) ELISA Kit is a double monoclonal sandwich enzyme immunoassay for the quantitative measurement of human Leptin receptor in serum, plasma and tissue culture medium.
Standard Curve Range
1 to 32 ng/mL
Sensitivity
0.05 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 6.70%
Intra-Assay
CV% < 8.40%
Spike Recovery
92.70%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LEPR

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Antibody Coated Microtiter Strips
  2. Conjugate Solution
  3. Dilution Buffer
  4. Master Standard
  5. Quality Control HIGH
  6. Quality Control LOW
  7. Stop Solution
  8. Substrate Solution
  9. Wash Solution Conc. (10x)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Leptin R ELISA Kit (Colorimetric)

  • B219
  • CD295 antigen
  • CD295
  • DB
  • DKFZp686B1731
  • huB219
  • LEPR
  • LEP-R
  • Leptin R
  • leptin receptor
  • LeptinR
  • OB R
  • OB receptor
  • OB-R
  • OBRCD295

Background

Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Leptin R ELISA Kit (KA0027). (Showing 1 - 1 of 1 FAQ).

  1. Please, confirm me if your kit Leptin Receptor ELISA Kit catalog # KA0027, is valid to quantify human soluble leptin receptor. I'm very sorry, but I'm looking for one kit valid to quantify the human soluble leptin receptor (sLR).
    • Catalog # KA0027, LEPR (Human) ELISA Kit is a double monoclonal sandwich enzyme immunoassay for the quantitative measurement of human Leptin receptor in serum, plasma and tissue culture medium. Abnova, the company for which we distribute this product, indicated that the antibodies present in this ELISA were raised against the extracellular domain of Leptin Receptor. Thus, this ELISA should be capable of detecting soluble leptin receptor.

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Bioinformatics

Gene Symbol LEPR