LIN-28A Antibody (1086) - Azide and BSA Free

Western Blot: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of A. 30 ug human ESC whole cell lysate/extract 12 % SDS-PAGE Lin28A antibody [717] dilution: 1:1000.

Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: LIN28 protein stained by LIN28 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of U87 xenograft. Lin28A antibody dilution: 1:100.

Flow Cytometry: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [1086] dilution: 1:50

Western Blot: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of 30 ug whole cell lysate. A: Non-transfected HeLa lysates B: LIN28 transfected HeLa lysates 12% SDS PAGE diluted at 1:1000. .

Western Blot: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of 30 ug whole cell lysate. A: NT2D1 12% SDS PAGE diluted at 1:1000.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 1086
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LIN-28A Antibody (1086) - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human LIN-28A. The exact sequence is proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIN28A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for LIN-28A Antibody (1086) - Azide and BSA Free

• CSDD1
• CSDD1lin-28A
• FLJ12457
• lin-28 homolog (C. elegans)
• lin-28 homolog A (C. elegans)
• LIN28
• LIN-28
• LIN28A
• LIN-28A
• LIN28RNA-binding protein LIN-28
• Tex17
• ZCCHC1
• ZCCHC1protein lin-28 homolog A
• Zinc finger CCHC domain-containing protein 1
• zinc finger, CCHC domain containing 1

Background

lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIN28A