Loading Control (Simple Western) Antibody Pack

Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06858] - Simple Western performed with GAPDH antibody, 2275-PC, on Jurkat (human acute T cell leukemia), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1:500 dilution.

Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06858] - Simple Western performed with HSP60 antibody, AF1800, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.

Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06858] - Simple Western performed with beta-Actin antibody, NB600-503, on Jurkat (human acute T cell leukemia), HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1:50 dilution.

Simple Western: Loading Control (Simple Western) Antibody Pack [NBP3-06858] - Simple Western performed with monoclonal beta Tubulin antibody, NB600-936, on HeLa (human cervical cancer cell line), C2C12 (mouse myoblastoma) and C6 (rat glioma) at 1ug/ml dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal

Loading Control (Simple Western) Antibody Pack Summary

• Description: This pack contains 1 vial each of: 2275-PC-100 (100 uL), AF1800 (50 ug), NB600-503 (0.05 mL), NB600-936 (0.1 mL).
• Clonality: Polyclonal

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Simple Western
  • Western Blot
• Application Notes: See individual datasheets of components for their validated applications
• Control:

  Jurkat Whole Cell Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

See individual datasheets of components for their validated species

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Loading Control (Simple Western) Antibody Pack

• GAPDH antibody
• Hsp60

Background

This loading control antibody pack has been designed specifically for optimization with Protein Simple's Simple Western Instruments, Jess and Wes. It includes rabbit polyclonal antibodies manufactured by Bio-Techne brands R&D Systems and Novus Biologicals. The polyclonal antibodies includes these targets: GAPDH, beta-actin, alpha tubulin and hsp60.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.