LPS from E. Coli, TLR4 ligand

• Applications: Func
• Concentration: LYOPH

LPS from E. Coli, TLR4 ligand Summary

• Description: This product is purified by Phenol extraction
  Appearance (Color): Clear to semi-yellow liquid
  Solubility (Solvent): Water
  Solubility (Conc): 4.90 - 5.10 mg/ml
  Solubility (Turbidity) :Faint Hazy to Hazy
  Solubility (Color) :Colorless to Light Yellow
  Protein Content (Method) :Lowry
  Prot. Content (% Protein): < 3.00 %
  Potency (Sample EU/mg): > 500000 EU/mg
  Lipopolysaccharide compounds are highly pyrogenic. Avoid inhalation of any LPS and prevent these compounds from entering the bloodstream
• Immunogen: Lipopolysaccharides from Escherichia coli 0111:B4 Gamma-irradiated, BioXtra, suitable for cell culture
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Functional
• Application Notes: Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). Use in Functional reported in scientific literature (PMID: 26121241)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: Ion exchange chromatography
• Reconstitution Instructions: Reconstitute with 1 mL sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations.

Notes

This product is purified by Phenol extraction
Appearance (Color): Clear to semi-yellow liquid
Solubility (Solvent): Water
Solubility (Conc): 4.90 - 5.10 mg/ml
Solubility (Turbidity) :Faint Hazy to Hazy
Solubility (Color) :Colorless to Light Yellow
Protein Content (Method) :Lowry
Prot. Content (% Protein): < 3.00 %
Potency (Sample EU/mg): > 500000 EU/mg
Lipopolysaccharide compounds are highly pyrogenic. Avoid inhalation of any LPS and prevent these compounds from entering the bloodstream

Alternate Names for LPS from E. Coli, TLR4 ligand

• L P S
• LPS from E. Coli TLR4 ligand
• LPS from E. COLI
• LPS
• TLR Ligand
• TLR4 Ligand

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for LPS from E. Coli, TLR4 ligand (NBP2-25295)(10)

Publications using NBP2-25295

• Liu Y, Diamond SL. Activation of Most Toll-Like Receptors in Whole Human Blood Attenuates Platelet Deposition on Collagen under Flow Journal of Immunology Research 2023-01-17 [PMID: 36703865]
• H Asashima, S Mohanty, M Comi, WE Ruff, KB Hoehn, P Wong, J Klein, C Lucas, I Cohen, S Coffey, N Lele, L Greta, K Raddassi, O Chaudhary, A Unterman, B Emu, SH Kleinstein, RR Montgomery, A Iwasaki, CS Dela Cruz, N Kaminski, AC Shaw, DA Hafler, TS Sumida PD-1highCXCR5-CD4+ peripheral helper T�cells promote CXCR3+ plasmablasts in human acute viral infection Cell Reports, 2023-01-02;0(0):111895. 2023-01-02 [PMID: 36596303]
• Ishida Y, Ohta K, Naruse T et al. Candida albicans b-glucan-containing particles increase HO-1 expression in oral keratinocytes via ROS/p38MAPK/Nrf2 pathway Infect. Immun. 2018-01-08 [PMID: 29311246] (Func)
• Kidana K, Tatebe T, Ito K et al. Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice EMBO Mol Med 2018-01-08 [PMID: 29311134] (Func)
• Kuen J, Darowski D, Kluge T, Majety M. Pancreatic cancer cell/fibroblast co-culture induces M2 like macrophages that influence therapeutic response in a 3D model PLoS ONE 2017-07-27 [PMID: 28750018] (Func, Human)
• Nakamura M, Kanda T, Sasaki R et al. MicroRNA-122 Inhibits the Production of Inflammatory Cytokines by Targeting the PKR Activator PACT in Human Hepatic Stellate Cells. PLoS ONE. 2015-12-05 [PMID: 26636761]
  
  Details:
  LPS from E. Coli, TLR4 ligand was used at 100 ng/mL concentration for the stimulation of hepatic stellate cells (LX-2 cells) and the stimulation treatment was done for 24 hours.
• Uraki S, Tameda M, Sugimoto K et al. Substitution in Amino Acid 70 of Hepatitis C Virus Core Protein Changes the Adipokine Profile via Toll-Like Receptor 2/4 Signaling PLoS ONE. 2015-06-30 [PMID: 26121241] (Func)
• Gillaux C, Mehats C, Vaiman D et al. Functional screening of TLRs in human amniotic epithelial cells. J Immunol. 2011-09-01 [PMID: 21775685]
  
  Details:
  TLR ligands: TLR1/2 (IMG-2201), TLR3 (IMG-2203), TLR4 (IMG-2204), TLR5 (IMG-2205), TLR6/2 (IMG-2206), TLR7 (IMG-2207), TLR9 (IMG-2209Hpt). The effects of ligand stimulation was measured by various readout assays, refer to the figures for details (Figs 2-8, S1).
• Kettenburg G Developing a Model of H5N1 Influenza Pathogenesis in Precision-Cut Human Lung Slices Thesis 2020-01-01
• Zhang S, Yuan B, Lam JH et al. Structure of the full-length human Pannexin1 channel and insights into its role in pyroptosis Cell Discovery 2021-12-01 [PMID: 33947837]

Review for LPS from E. Coli, TLR4 ligand (NBP2-25295) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Walter Jackson 02/26/2021

Summary

• Lot: 0000084279

Comments

• Comments: It worked well in the polarization and differentiation of mouse M0 macrophages into M1 macrophages in vitro.

FAQs for LPS from E. Coli, TLR4 ligand (NBP2-25295). (Showing 1 - 1 of 1 FAQs).

1. I want to use flow cytometry to quantify dendritic cells in lung tissue. Do you know the protocol for using LPS NBP2-25295 as a stimulant for dendritic cells? Is it similar to an overnight stimulation of PMA/ionomycin for T cells?
   • For this product we suggest looking at the publications to see what they did. The publication listed used 10ug/ml and incubated for 12hrs.