LRIG1 Antibody [Phycoerythrin]

D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse LRIG1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3688P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Phycoerythrin

LRIG1 Antibody [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse LRIG1
  Ala37-Thr794
  Accession # P70193
• Specificity: Detects mouse LRIG1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human LRIG3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Lrig1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRIG1 Antibody [Phycoerythrin]

• D6Bwg0781e
• Img
• LIG1
• LIG-1
• LRIG1

Background

LRIG1 (leucine-rich repeats and Ig-like domains-1; also LIG-1) is an approximately 130-145 kDa glycoprotein that belongs to the LRIG gene family.  It is widely expressed, and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and neurons.  LRIG1 is believed to negatively regulate the ErbB family of receptors.  In particular, and in a ligand-independent manner, LRIG1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number.  Alternatively, LRIG1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction.  Mature mouse LRIG1 is a 1057 amino acid (aa) type I transmembrane protein.  It contains a large 762 amino acid (aa) extracellular domain (ECD) (aa 35-795) plus a 274 aa cytoplasmic region.  The ECD contains 17 LRRs (aa’s 35-491) and three C2-type Ig-like domains (aa’s 497-781).  These two domain types are each sufficient for EGFR binding.  There one potential alternative splice form that a deletion of aa 875-923.  The LRIG1 ECD undergoes proteolysis, generating 90-105 and 60-70 kDa soluble fragments.  Over aa 37-794, human LRIG1 shares 97% and 90% aa sequence identity with rat and human LRIG1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRIG1 Antibody (FAB3688P)(1)

Publication using FAB3688P

• Wong , Vivian W, Stange , Daniel E, Page , Mahalia, Buczacki , Simon, Wabik , Agnieszk, Itami , Satoshi, van de Wetering , Marc, Poulsom , Richard, Wright , Nicholas, Trotter , Matthew, Watt , Fiona M, Winton , Doug J, Clevers , Hans, Jensen , Kim B Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling. Nat Cell Biol, 2012-03-04;14(4):401-8. 2012-03-04 [PMID: 22388892] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Lrig1
• Uniprot:
  • P70193()