LRRC15 Antibody (2944A) [Unconjugated]

HEK293 cells transfected with Human LRRC-15 and eGFP were stained with eGFP and either (A) Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LRRC15 at approximately 104 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of Human skin and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LRRC15 at approximately ~70-80kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow
• Clone: 2944A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

LRRC15 Antibody (2944A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human LRRC15
  Met1-Gly538
  Accession # Q8TF66
• Specificity: Detects human LRRC15 in direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRRC15 Antibody (2944A) [Unconjugated]

• hLib
• leucine rich repeat containing 15
• LIB
• LRRC15

Background

LRRC15 is a type-I 15-leucine-rich repeat containing membrane protein that is located on chromosome 3 at 3q29.  It is a 581 aa membrane protein that belong to the LRR superfamily and lacks an intracellular signaling domain.  Reports indicate that it is highly expressed on cancer-associated fibroblasts in numerous solid tumors, and in the epithelial cancer cell compartment of glioblastoma, sarcomas and melanoma tumors.  LRRC15 functions by regulating cell-cell and cell-extracellular matrix (ECM) interactions, likely partnering with fibronectin, laminin and collagen IV through its extracellular leucine-rich repeats.  LRRC15 is reported in multiple solid tumor types including breast, ovarian and cervical cancers where it exhibits Increased stromal expression (1).  Recent studies indicate that LRRC15 is co-expressed with COL10A1, a gene that is overexpressed in many types of solid tumors, including breast cancer (2).

1. Cancer Res. 2022 March 15; 82(6): 1038. doi:10.1158/0008-5472.CAN-21-0622. 
2. Biosci Rep. 2020 Feb 28; 40(2): BSR20193286. doi: 10.1042/BSR20193286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8TF66()