LXR alpha/NR1H3 Antibody (3E9) - Azide and BSA Free

Western Blot: LXR alpha/NR1H3 Antibody (3E9) [H00010062-M08] - Analysis of NR1H3 expression in transfected 293T cell line by NR1H3 monoclonal antibody (M08), clone 3E9. Lane 1: NR1H3 transfected lysatE (45.7 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: LXR alpha/NR1H3 Antibody (3E9) [H00010062-M08] - Detection limit for recombinant GST tagged NR1H3 is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LXR alpha/NR1H3 Antibody (3E9) - Azide and BSA Free Summary

• Immunogen: NR1H3 (AAH08819, 1 a.a. ~ 387 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSLWLGAPVPDIPPDSAVELWKPGAQDASSQAQGGSSCILREEARMPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR1H3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is useful for ELISA, Western Blot

Reactivity Notes

This product is reactive against Human.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LXR alpha/NR1H3 Antibody (3E9) - Azide and BSA Free

• alpha
• Liver X receptor alpha
• liver X receptor-alpha
• LXR alpha
• LXRA
• NR1H3
• Nuclear receptor subfamily 1 group H member 3
• nuclear receptor subfamily 1, group H, member 3
• oxysterols receptor LXR-alpha
• RLD-1

Background

Liver X receptors (LXRs), also known as RLD1, are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous. Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LXR alpha/NR1H3 Antibody (H00010062-M08)(2)

Publications using H00010062-M08

• Ménégaut L, Jalil A, Pilot T, van Dongen K et Al. Regulation of glycolytic genes in human macrophages by oxysterols: a potential role for liver X receptors Br J Pharmacol 2020-12-30 [PMID: 33377180]
• Ishibashi M, Filomenko R, Rebe C et al. Knock-down of the oxysterol receptor LXRalpha impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRbeta activation. Biochem Pharmacol. 2013-07-01 [PMID: 23313547] (WB, Human)

Bioinformatics

• Gene Symbol: NR1H3
• Uniprot:
  • AAH08819(Human)