Ly-6A/E Antibody (JM53-11)

Western Blot: Ly-6A/E Antibody (JM53-11) [NBP2-66939] - Analysis of LY6A on mouse brain tissue lysates using anti-LY6A antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Ly-6A/E Antibody (JM53-11) [NBP2-66939] - Analysis of paraffin-embedded mouse kidney tissue using anti-LY6A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ly-6A/E Antibody (JM53-11) [NBP2-66939] - Analysis of paraffin-embedded brain mouse tissue using anti-LY6A antibody. Counter stained with hematoxylin.

Product Discontinued

Ly-6A/E Antibody (JM53-11) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Mouse Ly-6A/E aa 41-90 / 134. (SwissProt: P05533 Mouse)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LY6A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ly-6A/E Antibody (JM53-11)

• Ly 6A/E
• Ly6A.2
• Ly6A/E
• Ly6E.1
• Lymphocyte antigen 6 complex locus A
• Lymphocyte antigen 6 complex locus E
• Retinoic acid induced gene E
• RIGE
• SCA1
• SCA-1
• SCA2
• Stem cell antigen 1
• Stem cell antigen 2
• T cell activating protein
• TAP
• Thymic shared antigen 1
• TSA1

Background

Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. (1-3) It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. (4-6) In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. (1, 7-9) The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. (1, 8, 9) Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses, (9-12) and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. (3, 13)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LY6A