Ly-6B.2 Antibody (7/4) - BSA Free

Immunocytochemistry/Immunofluorescence: Ly-6B.2 Antibody (7/4) [NBP2-13077] - IF staining of mouse Peyer's patch with Ly-6B.2 Antibody (7/4), NBP2-13077, green; counterstained for macrophages (F4/80), red and nuclei, blue.

Immunohistochemistry-Paraffin: Ly-6B.2 Antibody (7/4) [NBP2-13077] - Ly-6B.2 immunoreactivity in mouse bone marrow. Primary antibody was used at 125 ng/mL and was incubated with tissue sections for 30 minutes at room temperature. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Frozen: Ly-6B.2 Antibody (7/4) [NBP2-13077] - Staining of C57BL6 mouse spleen cryosection with Ly-6B.2 antibody (7/4).

• Reactivity: Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 7/4
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Ly-6B.2 Antibody (7/4) - BSA Free Summary

• Description: Fusion Partners Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
• Immunogen: Cultured bone marrow cells
• Specificity: Recognizes Ly6B.2, a polymorphic 40 kDa antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for 7/4:129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains Negative/Weak for 7/4:A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50 - 1:100
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly-6B.2 Antibody (NBP2-13077)(23)

Publications using NBP2-13077

• Ines Köhler, Cecilia Bivik Eding, Nada-Katarina Kasic, Deepti Verma, Charlotta Enerbäck NOS2-derived low levels of NO drive psoriasis pathogenesis Cell Death & Disease 2024-06-26 [PMID: 38926337]
• Janke LJ, Rehg JE The many faces of mouse histiocytic sarcoma in C57BL/6J mice Veterinary pathology 2023-05-03 [PMID: 37132518]
• Kaur G, Muthumalage T, Rahman I Clearance of senescent cells reverts the cigarette smoke-induced lung senescence and airspace enlargement in p16-3MR mice Aging cell 2023-04-20 [PMID: 37078230] (Immunohistochemistry-Frozen, Mouse)
• Lamb T, Muthumalage T, Meehan-Atrash J, Rahman I Nose-Only Exposure to Cherry- and Tobacco-Flavored E-Cigarettes Induced Lung Inflammation in Mice in a Sex-Dependent Manner Toxics 2022-08-13 [PMID: 36006150] (ICC/IF, Mouse)
  
  Details:
  ICC 1:250
• Cao S, Hung YW, Wang YC et al. Glutamine is essential for overcoming the immunosuppressive microenvironment in malignant salivary gland tumors Theranostics 2022-08-08 [PMID: 35966597] (FLOW, Mouse)
  
  Details:
  APC conjugated
• Karki R, Lee S, Mall R et al. ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection Science immunology 2022-05-19 [PMID: 35587515] (IHC-P, Mouse)
• Sharma S, Wang Q, Muthumalage T, Rahman I. Epithelial Ablation of Miro1/Rhot1 GTPase Augments Lung Inflammation by Cigarette Smoke Pathophysiology 2021-11-26 [PMID: 35366248] (Flow Cytometry)
• Millard SM, Heng O, Opperman KS Et al. Fragmentation of tissue-resident macrophages during isolation confounds analysis of single-cell preparations from mouse hematopoietic tissues Cell reports 2021-11-23 [PMID: 34818538]
• Eding CB, KOhler I, Verma D et al. MTH1 inhibitors for the treatment of psoriasis The Journal of investigative dermatology 2021-03-04 [PMID: 33676948] (IHC-P, Mouse)
• Wang Q, Sundar I, Li D et al. E-cigarette-Induced Pulmonary Inflammation and Dysregulated Repair are Mediated by nAChR ?7 Receptor: Role of nAChR ?7 in ACE2 Covid-19 receptor regulation Research Square 2022-11-07 [PMID: 32702718] (Flow Cytometry)
• Leal IV JM Crosstalk between Innate and Adaptive Immune Cells within Lymph Node Microenvironments Shapes Immunity to Vaccines Thesis 1905-07-13
• Wang Q, Sundar IK, Li D et al. E-cigarette-induced pulmonary inflammation and dysregulated repair are mediated by nAChR alpha 7 receptor: role of nAChR alpha 7 in SARS-CoV-2 Covid-19 ACE2 receptor regulation Respir. Res. 2020-06-18 [PMID: 32552811] (Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 format of this antibody.
• Tartey S, Gurung P, Karki R et Al. Ets-2 deletion in myeloid cells attenuates IL-1 alpha -mediated inflammatory disease caused by a Ptpn6 point mutation Cell. Mol. Immunol. 2020-03-19 [PMID: 32203187] (IHC-P, Mouse)
• Wang Q, Khan N A et al Dysregulated repair and inflammatory responses by e-cigarette-derived inhaled nicotine and humectant propylene glycol in a sex-dependent manner in mouse lung FASEB Bioadv 2019-12-12 [PMID: 31825014] (FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Albeituni S, Verbist KC, Tedrick PE et al. Mechanisms of Action of Ruxolitinib in Murine Models of Hemophagocytic Lymphohistiocytosis Blood 2019-04-23 [PMID: 31015190] (IF/IHC, Mouse)
• Rashid K, Sundar IK, Gerloff J et al. Lung cellular senescence is independent of aging in a mouse model of COPD/emphysema. Sci Rep 2018-06-13 [PMID: 29899396] (FLOW, Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Sundar IK, Rashid K, Gerloff J et al. Genetic ablation of histone deacetylase 2 leads to lung cellular senescence and lymphoid follicle formation in COPD/emphysema. FASEB J 2018-08-09 [PMID: 29630406] (Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Evrard M, Kwok IWH, Chong SZ, Teng KWW. Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity. 2018-02-20 [PMID: 29466759] (Mouse)
• Sundar IK, Rashid K, Gerloff J, Li D. Genetic Ablation of p16(Ink4a) Does Not Protect Against Cellular Senescence in Mouse Models of COPD/Emphysema. Am. J. Respir. Cell Mol. Biol. 2018-02-15 [PMID: 29447461] (Mouse)
  
  Details:
  This citation used the Alexa Fluor 488 form of this antbody
• Sundar IK, Rashid K, Sellix MT, Rahman I. The nuclear receptor and clock gene REV-ERBa regulates cigarette smoke-induced lung inflammation. Biochem. Biophys. Res. Commun. 2017-12-02 [PMID: 28974420] (WB, Mouse)
• Karmaus PWF, Herrada AA, Guy C et al. Critical roles of mTORC1 signaling and metabolic reprogramming for M-CSF-mediated myelopoiesis. J. Exp. Med. 2017-09-04 [PMID: 28784627] (IHC-P, Mouse)
• Kamei A, Gao G, Neale G et al. Exogenous remodeling of lung resident macrophages protects against infectious consequences of bone marrow-suppressive chemotherapy Proc. Natl. Acad. Sci. U.S.A. 2016-09-26 [PMID: 27671632] (IF/IHC, Mouse)
• Svedova J, Tsurutani N, Liu W. TNF and CD28 Signaling Play Unique but Complementary Roles in the Systemic Recruitment of Innate Immune Cells after Staphylococcus aureus Enterotoxin A Inhalation. J Immunol. 2016-04-20 [PMID: 27183621] (IHC-Fr, Mouse)
  
  Details:
  This publication used the DL488 conjugated form of this antibody (NBP2-13077G)

Review for Ly-6B.2 Antibody (NBP2-13077) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 11/01/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: bone marrow
• Species: Mouse
• Lot: 1803