Ly-6C Antibody (ER-MP20) [Biotin]

Immunohistochemistry: Ly-6C Antibody (ER-MP20) [Biotin] - [NB100-64203] - Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody. Low power

• Reactivity: Mu
• Applications: Flow
• Clone: ER-MP20
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: 0.1 mg/ml

Ly-6C Antibody (ER-MP20) [Biotin] Summary

• Immunogen: Balb/c macrophage precursor cell hybrids
• Specificity: Ly6C (ER-MP20)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly6c1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
• Application Notes: This antibody is useful for ELISA, Functional, Flow cytometry / FACS analysis, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% Bovine Serum Albumin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Ly-6C Antibody (ER-MP20) [Biotin]

• Ly 6c
• Ly6c protein
• Lymphocyte antigen 6 complex locus C
• Lymphocyte antigen 6 complex
• Lymphocyte antigen 6C
• Lymphocyte antigen Ly 6C

Background

Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes. This antibody is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by Flow Cytometry. ab15674 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. This antibody also detects a wide range of endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ly6c1
• Entrez:
  • 17067(Mouse)
• Uniprot:
  • P0CW02(Mouse)
  • P0CW03(Mouse)