Ly-6C Antibody (ER-MP20) - BSA Free

Images

 
Immunohistochemistry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Staining of a mouse lymph node cryosection.
Flow Cytometry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Peripheral Mouse Granulocytes stained with Rat anti Mouse Ly-6C:RPE.
Immunohistochemistry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Staining of a mouse lymph node cryosection.
Immunohistochemistry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Staining of a mouse lymph node cryosection.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IP
Clone
ER-MP20
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Ly-6C Antibody (ER-MP20) - BSA Free Summary

Immunogen
Balb/c macrophage precursor cell hybrids
Specificity
Ly6C (ER-MP20)
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Ly6c1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Publications
Read Publication using
NB100-65413 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Ly-6C Antibody (ER-MP20) - BSA Free

  • Ly 6c
  • Ly6c protein
  • Lymphocyte antigen 6 complex locus C
  • Lymphocyte antigen 6 complex
  • Lymphocyte antigen 6C
  • Lymphocyte antigen Ly 6C

Background

Ly-6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes. Using ER-MP20, distinct mouse blood monocyte subsets can be distinguished: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ly-6C Antibody (NB100-65413)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
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Showing Publication 1 - 1 of 1.
Publication using NB100-65413 Applications Species
Hreha T, Collins C, Hunstad D Androgen exposure impairs neutrophil maturation and function within the infected kidney bioRxiv 2023-06-24 (Flow Cytometry, Mouse) Flow Cytometry Mouse

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Bioinformatics

Gene Symbol Ly6c1
Entrez
Uniprot