Lysozyme Antibody (LYZ/3944)

Western Blot: Lysozyme Antibody (LYZ/3944) [NBP3-13962] - Western blot analysis of human spleen tissue and HepG2 cell lysates using Lysozyme antibody (LYZ/3944).

Western Blot: Lysozyme Antibody (LYZ/3944) [NBP3-13962] - Western blot analysis of human spleen tissue and HepG2 cell lysates using Lysozyme antibody (LYZ/3944).

Immunohistochemistry-Paraffin: Lysozyme Antibody (LYZ/3944) [NBP3-13962] - Formalin-fixed, paraffin-embedded human spleen stained with Lysozyme antibody (LYZ/3944). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: WB, IHC, MA
• Clone: LYZ/3944
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Lysozyme Antibody (LYZ/3944) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14115)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant fragment of human Lysozyme protein (around aa 18-147) (exact sequence is proprietary) (Uniprot: P61626)
• Localization: Secreted
• Marker: Histiocytoma & Monocytic Acute Leukemia Marker
• Specificity: Lysozyme is an enzyme, commonly referred to as the body s own antibiotic since it kills bacteria. Natural substrate of lysozyme is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Lysozyme is synthesized predominantly in reactive histiocytes rather than in resting, unstimulated phagocytes. This antibody labels myeloid cells, histiocytes, granulocytes, macrophages and monocytes. It is helpful in the identification of myeloid or monocytic nature of acute leukemia.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LYZ
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Lysozyme Antibody (LYZ/3944)

• EC 3.2.1.17
• lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C
• lysozyme C
• Lysozyme
• LZM
• renal amyloidosis

Background

Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N acetylmuramic acid and N acetylglucosamine. It is found in spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LYZ
• Uniprot:
  • P61626(Human)