LYVE-1 Antibody (223322) [Unconjugated]

Western blot shows lysate of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LYVE-1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE‑1 Monoclonal Antibody (Catalog # MAB2125, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

LYVE-1 was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse LYVE-1 (positive control) and wild type NS0 mouse myeloma cell line (negative control) using Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

LYVE-1 was detected in immersion fixed frozen sections of mouse intestine tissue using Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in lymphatic vessels. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready, ICC/IF
• Clone: 223322
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

LYVE-1 Antibody (223322) [Unconjugated] Summary

• Immunogen: BaF/3 mouse pro-B cell line transfected with mouse LYVE-1
  Ala24-Thr234
  Accession # Q8BHC0
• Specificity: Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LYVE1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LYVE-1 Antibody (223322) [Unconjugated]

• cell surface retention sequence binding protein-1
• Cell surface retention sequence-binding protein 1
• CRSBP1
• CRSBP-1
• extracellular link domain containing 1
• extracellular link domain-containing 1
• Extracellular link domain-containing protein 1
• HAR
• Hyaluronic acid receptor
• lymphatic vessel endothelial hyaluronan receptor 1
• lymphatic vessel endothelial hyaluronic acid receptor 1
• LYVE1
• LYVE-1
• UNQ230/PRO263
• XLKD1

Background

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
2. Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1.
3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
6. Carreira, C.M. et al. (2001) Cancer Research 61:8079.
7. Jackson, D.J. et al. (2001) Trends Immunol. 22:317.
8. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
9. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
10. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
11. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LYVE-1 Antibody (MAB2125)(41)

Publications using MAB2125

• Park, C;Hwang, IY;Yan, SL;Vimonpatranon, S;Wei, D;Van Ryk, D;Girard, A;Cicala, C;Arthos, J;Kehrl, JH; Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage eLife 2024-03-20 [PMID: 38507462] (Immunohistochemistry, Mouse)
• Uçar, MC;Hannezo, E;Tiilikainen, E;Liaqat, I;Jakobsson, E;Nurmi, H;Vaahtomeri, K; Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks Nature communications 2023-09-21 [PMID: 37735168] (IHC, Mouse)
• Dong Dong, Heng Wang, Liang Chen, Wei Wang, Tianyi Liu Hormone Therapy: A Potential Risk Factor Affecting Survival and Functional Restoration of Transplanted Lymph Nodes Frontiers in Pharmacology 3/31/2022 [PMID: 35431925]
• Peter Baluk, Donald M. McDonald Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts Methods in Molecular Biology 2022-09-06 [PMID: 35099733]
• Rafael S. Czepielewski, Emma C. Erlich, Emily J. Onufer, Shannon Young, Brian T. Saunders, Yong-Hyun Han et al. Ileitis-associated tertiary lymphoid organs originate at lymphatic valves and obstruct mesenteric lymph flow in response to tumor necrosis factor Immunity 2021-12-01 [PMID: 34788601]
• E Redder, N Kirschnick, S Bobe, R Hägerling, NR Hansmeier, F Kiefer Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities PLoS ONE, 2021-09-20;16(9):e0249256. 2021-09-20 [PMID: 34543279] (IHC, Mouse)
• Grzegorz Gula, Sławomir Rumiński, Justyna Niderla-Bielińska, Agnieszka Jasińska, Ewelina Kiernozek, Ewa Jankowska-Steifer et al. Potential functions of embryonic cardiac macrophages in angiogenesis, lymphangiogenesis and extracellular matrix remodeling Histochemistry and Cell Biology 2021-01-01 [PMID: 33130914]
• Maike Frye, Simon Stritt, Henrik Ortsäter, Magda Hernandez Vasquez, Mika Kaakinen, Andres Vicente et al. EphrinB2-EphB4 signalling provides Rho-mediated homeostatic control of lymphatic endothelial cell junction integrity eLife 2020-09-08 [PMID: 32897857]
• SP Hong, MJ Yang, H Cho, I Park, H Bae, K Choe, SH Suh, RH Adams, K Alitalo, D Lim, GY Koh Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi Nat Commun, 2020-08-14;11(1):4102. 2020-08-14 [PMID: 32796823] (ELISA Detection, Labeling, Mouse)
• L Li, MW Shirkey, T Zhang, Y Xiong, W Piao, V Saxena, C Paluskievi, YS Lee, N Toney, BM Cerel, Q Li, T Simon, KD Smith, KL Hippen, BR Blazar, R Abdi, JS Bromberg The lymph node stromal laminin alpha 5 shapes alloimmunity J. Clin. Invest., 2020-05-01;0(0):. 2020-05-01 [PMID: 32017712] (IHC, Mouse)
• X Jia, F Gábris, Ó Jacobsen, G Bedics, B Botz, Z Helyes, Z Kellermaye, D Vojkovics, G Berta, N Nagy, Z Jakus, P Balogh Foliate Lymphoid Aggregates as Novel Forms of Serous Lymphocyte Entry Sites of Peritoneal B Cells and High-Grade B Cell Lymphomas J. Immunol., 2019-11-25;0(0):. 2019-11-25 [PMID: 31767783] (Flow Cytometry, Mouse)
• Stella J. Berendam, Alexander F. Koeppel, Nicole R. Godfrey, Sherin J. Rouhani, Amber N. Woods, Anthony B. Rodriguez et al. Comparative Transcriptomic Analysis Identifies a Range of Immunologically Related Functional Elaborations of Lymph Node Associated Lymphatic and Blood Endothelial Cells Frontiers in Immunology 2019-04-16 [PMID: 31057546]
• K Veerman, C Tardiveau, F Martins, J Coudert, JP Girard Single-Cell Analysis Reveals Heterogeneity of High Endothelial Venules and Different Regulation of Genes Controlling Lymphocyte Entry to Lymph Nodes Cell Rep, 2019-03-12;26(11):3116-3131.e5. 2019-03-12 [PMID: 30865898] (ICC, Mouse)
• J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells PLoS ONE, 2018-10-29;13(10):e0206756. 2018-10-29 [PMID: 30372497] (Flow Cytometry, Mouse)
• Peter Baluk, Donald M. McDonald Imaging Lymphatics in Mouse Lungs Methods in Molecular Biology 2019-04-29 [PMID: 30242759]
• M Ouarné, C Bouvard, G Boneva, C Mallet, J Ribeiro, A Desroches-, E Soleilhac, E Tillet, O Peyruchaud, S Bailly BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer J. Exp. Clin. Cancer Res., 2018-08-30;37(1):209. 2018-08-30 [PMID: 30165893] (IHC, Mouse)
• M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program Nat Commun, 2018-04-17;9(1):1511. 2018-04-17 [PMID: 29666442] (IHC, Mouse)
• Y Zhang, MH Ulvmar, L Stanczuk, I Martinez-C, M Frye, K Alitalo, T Mäkinen Heterogeneity in VEGFR3 levels drives lymphatic vessel hyperplasia through cell-autonomous and non-cell-autonomous mechanisms Nat Commun, 2018-04-03;9(1):1296. 4/3/2018 [PMID: 29615616]
• LK Beura, S Wijeyesing, EA Thompson, MG Macchietto, PC Rosato, MJ Pierson, JM Schenkel, JS Mitchell, V Vezys, BT Fife, S Shen, D Masopust T Cells in Nonlymphoid Tissues Give Rise to Lymph-Node-Resident Memory T Cells Immunity, 2018-02-20;48(2):327-338.e5. 2018-02-20 [PMID: 29466758] (IHC-Fr, Mouse)
• Salli Antila, Sinem Karaman, Harri Nurmi, Mikko Airavaara, Merja H. Voutilainen, Thomas Mathivet et al. Development and plasticity of meningeal lymphatic vessels Journal of Experimental Medicine 2017-12-04 [PMID: 29141865]
• T Torcellan, HR Hampton, J Bailey, M Tomura, R Brink, T Chtanova In vivo photolabeling of tumor-infiltrating cells reveals highly regulated egress of T-cell subsets from tumors Proc. Natl. Acad. Sci. U.S.A., 2017-05-15;0(0):. 2017-05-15 [PMID: 28507145] (IHC-Fr, Mouse)
• LA Johnson, S Banerji, W Lawrance, U Gileadi, G Prota, KA Holder, YM Roshorm, T Hanke, V Cerundolo, NW Gale, DG Jackson Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1 Nat. Immunol., 2017-05-15;0(0):. 2017-05-15 [PMID: 28504698] (Neutralization, Human)
• Tianliang Sun A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib J. Cell Biol, 2016-12-22;0(0):. 2016-12-22 [PMID: 28007914] (ICC, Mouse)
• Tsun-Ho Lo Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration J Immunol, 2016-06-17;0(0):. 2016-06-17 [PMID: 27316686] (IHC-Fr, Mouse)
• Wang J, Shah D, Chen X, Anderson R, Wu M A micro-sterile inflammation array as an adjuvant for influenza vaccines. Nat Commun, 2014-07-18;5(0):4447. 2014-07-18 [PMID: 25033973] (IHC, Mouse)
• Wu M, Du Y, Liu Y, He Y, Yang C, Wang W, Gao F Low molecular weight hyaluronan induces lymphangiogenesis through LYVE-1-mediated signaling pathways. PLoS ONE, 2014-03-25;9(3):e92857. 2014-03-25 [PMID: 24667755] (Neutralization, Mouse)
• Dartsch N, Schulte D, Hagerling R, Kiefer F, Vestweber D Fusing VE-cadherin to alpha-catenin impairs fetal liver hematopoiesis and lymph but not blood vessel formation. Mol Cell Biol, 2014-02-24;34(9):1634-48. 2014-02-24 [PMID: 24567373] (ICC, Mouse)
• Thorek D, Ulmert D, Diop N, Lupu M, Doran M, Huang R, Abou D, Larson S, Grimm J Non-invasive mapping of deep-tissue lymph nodes in live animals using a multimodal PET/MRI nanoparticle. Nat Commun, 2014-01-01;5(0):3097. 2014-01-01 [PMID: 24445347] (IHC, Mouse)
• Qiu Guo, Yi Ke Xu, Ke Ke Ren, Ke WenGe Sun, WenGe Yi Liu Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles International Journal of Nanomedicine 2013-07-03 [PMID: 23818783]
• Sandrine Levet, Delphine Ciais, Galina Merdzhanova, Christine Mallet, Teresa A. Zimmers, Se-Jin Lee et al. Bone morphogenetic protein 9 (BMP9) controls lymphatic vessel maturation and valve formation Blood 2013-07-25 [PMID: 23741013]
• Reichardt , Peter, Patzak , Irene, Jones , Kristian, Etemire , Eloho, Gunzer , Matthias, Hogg , Nancy A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes. EMBO J, 2013-02-26;32(6):829-43. 2013-02-26 [PMID: 23443048] (IHC, Mouse)
• Zhi L, Kim P, Thompson BD, Pitsillides C, Bankovich AJ, Yun SH, Lin CP, Cyster JG, Wu MX FTY720 blocks egress of T cells in part by abrogation of their adhesion on the lymph node sinus. J. Immunol., 2011-07-25;187(5):2244-51. 2011-07-25 [PMID: 21788441] (Intravital Microscopy, Intravital Microscopy, Mouse)
• Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. J. Clin. Invest., 2009-09-14;119(10):2954-64. 2009-09-14 [PMID: 19759514] (IHC-Fr, Mouse)
• Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth. Proc. Natl. Acad. Sci. U.S.A., 2009-04-09;106(17):7137-42. 2009-04-09 [PMID: 19359485] (Flow Cytometry, Mouse)
• Sinha RK, Park C, Hwang IY, Davis MD, Kehrl JH B lymphocytes exit lymph nodes through cortical lymphatic sinusoids by a mechanism independent of sphingosine-1-phosphate-mediated chemotaxis. Immunity, 2009-02-19;30(3):434-46. 2009-02-19 [PMID: 19230723] (Neutralization, Mouse)
• Singh S, Bond VC, Powell M, Singh UP, Bumpers HL, Grizzle WE, Lillard JW CXCR4-gp120-IIIB interactions induce caspase-mediated apoptosis of prostate cancer cells and inhibit tumor growth. Mol. Cancer Ther., 2009-01-01;8(1):178-84. 2009-01-01 [PMID: 19139127] (IHC-P, Mouse)
• Grigorova IL, Schwab SR, Phan TG, Pham TH, Okada T, Cyster JG Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells. Nat. Immunol., 2008-12-07;10(1):58-65. 2008-12-07 [PMID: 19060900] (IHC-Fr, Neutralization, Mouse)
• Pham TH, Okada T, Matloubian M, Lo CG, Cyster JG S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress. Immunity, 2007-12-27;28(1):122-33. 2007-12-27 [PMID: 18164221] (Flow Cytometry, IHC-Fr, Mouse)
• Kabashima K, Shiraishi N, Sugita K, Mori T, Onoue A, Kobayashi M, Sakabe J, Yoshiki R, Tamamura H, Fujii N, Inaba K, Tokura Y CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells. Am. J. Pathol., 2007-09-06;171(4):1249-57. 2007-09-06 [PMID: 17823289] (IHC-Fr, Mouse)
• Phan TG, Grigorova I, Okada T, Cyster JG Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells. Nat. Immunol., 2007-07-29;8(9):992-1000. 2007-07-29 [PMID: 17660822] (IHC, Mouse)
• Backhed F, Crawford PA, O&amp;apos;Donnell D, Gordon JI Postnatal lymphatic partitioning from the blood vasculature in the small intestine requires fasting-induced adipose factor. Proc. Natl. Acad. Sci. U.S.A., 2007-01-03;104(2):606-11. 2007-01-03 [PMID: 17202268] (IHC, Mouse)

Bioinformatics

• Gene Symbol: LYVE1
• Uniprot:
  • Q8BHC0()