Macrophage Antibody (MOMA-2) - BSA Free

Immunocytochemistry/Immunofluorescence: Macrophage Antibody (MOMA-2) [NB100-64946] - Cabanel M, Brand C, Oliveira-Nunes MC, Cabral-Piccin MP, Lopes MF, Brito JM, et al. (2015) Epigenetic Control of Macrophage Shape Transition towards an Atypical Elongated Phenotype by Histone Deacetylase Activity. PLoS ONE 10(7): e0132984.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Benard A, Sala C, Pluschke G (2016)Mycobacterium ulcerans Mouse Model Refinement for Pre-Clinical Profiling of Vaccine Candidates.PLoS ONE 11(11): e0167059

Flow Cytometry: Macrophage Antibody (MOMA-2) [NB100-64946] - Staining of mouse peritoneal macrophages with RPE conjugated Rat anti Mouse Macrophages/Monocytes following permeabilisation with Leucoperm

Immunocytochemistry/Immunofluorescence: Macrophage Antibody (MOMA-2) [NB100-64946] - Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.PLoS Pathog 3(12): e193.

Immunocytochemistry/Immunofluorescence: Macrophage Antibody (MOMA-2) [NB100-64946] - Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.

Immunocytochemistry/Immunofluorescence: Macrophage Antibody (MOMA-2) [NB100-64946] - Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.PLoS Pathog 3(12): e193.

Immunocytochemistry/Immunofluorescence: Macrophage Antibody (MOMA-2) [NB100-64946] - Dong M, Zhong L, Chen WQ, Ji XP, Zhang M, Zhao YX, et al. (2012) Doxycycline Stabilizes Vulnerable Plaque via Inhibiting Matrix Metalloproteinases and Attenuating Inflammation in Rabbits.PLoS ONE 7(6): e39695.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Marie-Claude Bourdillon, Jacques Randon, Lydie Barek, et al.,

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Ruschke K, Ebelt H, Kloting N, Boettger T, Raum K, Bluher M, et al. (2009) Defective Peripheral Nerve Development Is Linked to Abnormal Architecture and Metabolic Activity of Adipose Tissue in Nscl-2 Mutant Mice.PLoS ONE 4(5): e5516.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Seno T, Hamaguchi M, Ashihara E, Kohno M, Ishino H, Yamamoto A, et al. (2011) 15-Deoxy-delta 12,14 Prostaglandin J2 Reduces the Formation of Atherosclerotic Lesions in Apolipoprotein E Knockout Mice. PLoS ONE 6(10): e25541.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Chyu K-Y, Zhao X, Dimayuga PC, Zhou J, Li X, Yano J, et al. (2012) CD8+ T Cells Mediate the Athero-Protective Effect of Immunization with an ApoB-100 Peptide.PLoS ONE 7(2): e30780.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Wang B, Dai S, Dong Z, Sun Y, Song X, Guo C, et al. (2014) The Modulation of Endoplasmic Reticulum Stress by Chemical Chaperone Upregulates Immune Negative Cytokine IL-35 in Apolipoprotein E-Deficient Mice.PLoS ONE 9(1): e87787.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Liu XL, Zhang PF, Ding SF, Wang Y, Zhang M, Zhao YX, et al. (2012) Local Gene Silencing of Monocyte Chemoattractant Protein-1 Prevents Vulnerable Plaque Disruption in Apolipoprotein E-Knockout Mice.PLoS ONE 7(3): e33497.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Chyu K-Y, Lio WM, Dimayuga PC, Zhou J, Zhao X, Yano J, et al. (2014) Cholesterol Lowering Modulates T Cell Function In Vivo and In Vitro. PLoS ONE 9(3): e92095.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Kennedy A, Gruen ML, Gutierrez DA, Surmi BK, Orr JS, Webb CD, et al. (2012) Impact of Macrophage Inflammatory Protein-1a Deficiency on Atherosclerotic Lesion Formation, Hepatic Steatosis, and Adipose Tissue Expansion.PLoS ONE 7(2): e31508.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Wang L, Yang M, Arias A, Song L, Li F, Tian F, et al. (2015) Splenocytes Seed Bone Marrow of Myeloablated Mice: Implication for Atherosclerosis. PLoS ONE 10(6): e0125961.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Niven J, Hoare J, McGowan D, Devarajan G, Itohara S, Gannage M, et al. (2015) S100B Up-Regulates Macrophage Production of IL1b and CCL22 and Influences Severity of Retinal Inflammation.PLoS ONE 10(7): e0132688.

Immunohistochemistry: Macrophage Antibody (MOMA-2) [NB100-64946] - Shuto Y, Asai A, Nagao M, Sugihara H, Oikawa S (2015) Repetitive Glucose Spikes Accelerate Atherosclerotic Lesion Formation in C57BL/6 Mice.PLoS ONE 10(8): e0136840.

Flow Cytometry: Macrophage Antibody (MOMA-2) [NB100-64946] - Staining of mouse peritoneal macrophages with FITC conjugated Rat anti Mouse Macrophages/Monocytes following permeabilisation with Leucoperm

• Reactivity: Mu
• Applications: Flow, IHC, KO
• Clone: MOMA-2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Macrophage Antibody (MOMA-2) - BSA Free Summary

• Immunogen: Mouse lymph node stroma
• Specificity: Recognizes an intracellular antigen of mouse macrophages and monocytes. It reacts strongly with macrophages in lymphoid organs in all mouse strains tested. Staining may also be seen on the epithelium of mucosa.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Knockout Validated

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: <0.1% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Macrophage Antibody (MOMA-2) - BSA Free

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MMIF
• Phenylpyruvate tautomerase

Background

Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Macrophage Antibody (NB100-64946)(1)

Publication using NB100-64946

• Klak M, Wszo?a M, Berman A et al. Bioprinted 3D Bionic Scaffolds with Pancreatic Islets as a New Therapy for Type 1 Diabetes-Analysis of the Results of Preclinical Studies on a Mouse Model Journal of functional biomaterials 2023-07-14 [PMID: 37504866] (IHC, Mouse)