Macrophage Antibody (RM0029-11H3) - Azide and BSA Free

Immunohistochemistry-Paraffin: Macrophage Antibody (RM0029-11H3) [NBP2-12436] - Mouse skin tissue from human breast cancer cell xenograft.

• Reactivity: Mu
• Applications: Flow, IHC, IP, CyTOF-ready
• Clone: RM0029-11H3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Macrophage Antibody (RM0029-11H3) - Azide and BSA Free Summary

• Immunogen: Isolated mouse macrophages
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for Macrophage Antibody (RM0029-11H3) - Azide and BSA Free

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MMIF
• Phenylpyruvate tautomerase

Background

Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Macrophage Antibody (NBP2-12436)(1)

Publication using NBP2-12436

• Lundh M, Petersen PS, Isidor MS et al. Afadin is a scaffold protein repressing insulin action via HDAC6 in adipose tissue EMBO Rep. 2019-07-02 [PMID: 31264358]