MAdCAM-1 Antibody (MECA-367) [FITC]

Immunohistochemistry-Paraffin: MAdCAM-1 Antibody (MECA-367) [FITC] [NBP1-28147] - Mouse intestine section was stained with Rat Anti-Mouse MAdCAM-1-UNLB followed by an HRP conjugated secondary antibody, DAB, and hematoxylin.

Immunohistochemistry: MAdCAM-1 Antibody (MECA-367) [FITC] [NBP1-28147] - Staining of BALB/c mouse intestine section with Biotin and FITC conjugated antibodies.

Product Discontinued

MAdCAM-1 Antibody (MECA-367) [FITC] Summary

• Immunogen: Endothelial cells isolated from BALB/c mesenteric and peripheral lymph nodes
• Specificity: Murine MAdCAM-1 (Mr 58-88 kDa)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: MADCAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for MAdCAM-1 Antibody (MECA-367) [FITC]

• hMAdCAM-1
• MACAM1
• MAdCAM1
• MAdCAM-1
• mucosal addressin cell adhesion molecule 1
• mucosal addressin cell adhesion molecule-1
• mucosal vascular addressin cell adhesion molecule 1

Background

Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer's patches and mesenteric lymph nodes, and on flat walled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen. (1-3) The countereceptor or 'homing receptor' for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin. (4, 5) The monoclonal antibody MECA-367 binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer's patch HEV in vivo. (1, 2, 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MADCAM1