MafB Antibody

Product Discontinued

MafB Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog B using the numbering given in entry NP_034788.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAFB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10-1:500
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Previous lot has only been tested on paraffin sections but previously used applications are expected to work. These applications include: Chromatin Immunoprecipitation, Western Blot and Immunohistochemistry - paraffin and frozen. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24327562)

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 26554594)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MafB Antibody

• Kreisler (mouse) maf-related leucine zipper homolog
• Kreisler maf-related leucine zipper homolog
• KRML
• KRMLMAFB/Kreisler basic region/leucine zipper transcription factor
• MafB
• Maf-B
• MGC43127
• transcription factor MafB
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
• V-maf musculoaponeurotic fibrosarcoma oncogene homolog B

Background

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MafB Antibody (NB600-266)(10)

Publications using NB600-266

• Alcantara MC, Suzuki K, Acebedo AR et al. Androgen-regulated MafB drives cell migration via MMP11-dependent extracellular matrix remodeling in mice iScience 2022-12-01 [PMID: 36465133] (Mouse)
• Russell R, Carnese PP, Hennings TG et al. Loss of the transcription factor MAFB limits beta-cell derivation from human PSCs Nat Commun 2020-06-02 [PMID: 32488111] (ICC/IF, Mouse)
• Pere-Vedrenne C, He W, Azzi-Martin L et Al. The Nuclear Remodeling Induced by Helicobacter Cytolethal Distending Toxin Involves MAFB Oncoprotein Toxins (Basel) 2020-03-12 [PMID: 32178359] (Human)
• Freeman S, Mateo SAnchez S, Pouyo R et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity EMBO Rep. 2019-07-19 [PMID: 31321879]
• Spaeth JM, Gupte M, Perelis M et al. Defining a Novel Role for the PDX1 Transcription Factor in Islet B Cell Maturation and Proliferation During Weaning Diabetes 2017-07-13 [PMID: 28705881] (Mouse)
• Park So Jeong, Huh Jeong-Eun, Shin Jihye et al. Sirt6 coopeRates with Blimp1 to positively regulate osteoclast differentiation. Scientific Reports 2016-01-01 [PMID: 27189179] (WB, WB, Mouse)
• Johnson NB, Posluszny JA, He LK et al. Perturbed MafB/GATA1 axis after burn trauma bares the potential mechanism for immune suppression and anemia of critical illness J. Leukoc. Biol. 2016-03-18 [PMID: 26992433] (WB, Mouse)
• Conrad E, Dai C, Spaeth J et al. The MAFB transcription factor impacts islet a-cell function in rodents and represents a unique signature of primate islet b-cells. Am. J. Physiol. Endocrinol. Metab. 2016-01-01 [PMID: 26554594] (ICC/IF, Primate, Human)
• Yu WM, Appler JM, Kim YH et al. A Gata3-Mafb transcriptional network directs post-synaptic differentiation in synapses specialized for hearing. Elife 2013-12-10 [PMID: 24327562] (ICC/IF, WB, IHC-Fr, Mouse)
• Jakobsen JS, Waage J, Rapin N et al. Temporal mapping of CEBPA and CEBPB binding during liver regeneration reveals dynamic occupancy and specific regulatory codes for homeostatic and cell cycle gene batteries Genome Res 2013-04-01 [PMID: 23403033] (Chemotaxis, Mouse)

Reviews for MafB Antibody (NB600-266) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 09/14/2015

Summary

• Application: Immunofluorescence
• Species: Mouse

reviewed by: Verified Customer IF Mouse 04/02/2015

Summary

• Application: Immunofluorescence
• Species: Mouse

reviewed by: Verified Customer ChIP Mouse 05/28/2013

Summary

• Application: Chromatin Immunoprecipitation
• Species: Mouse

FAQs for MafB Antibody (NB600-266). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration of NB600-266 lot #1  ..show answer..
   • The concentration of NB600-266 is 0.05 mg/ml

Bioinformatics

• Gene Symbol: MAFB
• Entrez:
  • 9935(Human)
  • 16658(Mouse)
• Uniprot:
  • P54841(Mouse)