Malondialdehyde Antibody (11E3) - BSA Free

Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.

Immunocytochemistry/Immunofluorescence: Malondialdehyde Antibody (11E3) [NBP2-59367] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 M H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Immunohistochemistry: Malondialdehyde Antibody (11E3) [NBP2-59367] - Immunohistochemistry analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 10X.

Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367] - Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 ug). Lane 3: Malondialdehyde-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug) . Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 11E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Malondialdehyde Antibody (11E3) - BSA Free Summary

• Immunogen: Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Malondialdehyde Antibody (11E3) - BSA Free

• 3-Propanedial;Malonaldehyde;Malondialdehyde;MDA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Malondialdehyde Antibody (NBP2-59367)(4)

Publications using NBP2-59367

• Yuan K, Yang Y, Lin Y et al. Targeting Bacteria‐Induced Ferroptosis of Bone Marrow Mesenchymal Stem Cells to Promote the Repair of Infected Bone Defects Advanced Science 2024-08-21 [PMID: 39166412]
• Belinda J Hernandez, Nikolai P Skiba, Karolina Plössl, Madison Strain, Yutao Liu, Daniel Grigsby, Una Kelly, Martha A Cady, Vikram Manocha, Arvydas Maminishkis, TeddiJo Watkins, Sheldon S Miller, Allison Ashley-Koch, W Daniel Stamer, Bernhard H F Weber, Catherine Bowes Rickman, Mikael Klingeborn Polarized Desmosome and Hemidesmosome Shedding via Small Extracellular Vesicles is an Early Indicator of Outer Blood-Retina Barrier Dysfunction. Journal of extracellular biology 2023-10-01 [PMID: 38108061]
• Focken J, Scheurer J, Jäger A et al. Neutrophil extracellular traps enhance S. aureus skin colonization by oxidative stress induction and downregulation of epidermal barrier genes Cell reports 2023-09-19 [PMID: 37733587] (IHC, Mouse)
• Song SH, Han D, Park K et al. Bone morphogenetic protein-7 attenuates pancreatic damage under diabetic conditions and prevents progression to diabetic nephropathy via inhibition of ferroptosis Frontiers in endocrinology 2023-05-24 [PMID: 37293506] (IHC-P, Mouse)