MAP2 Antibody (AP20)

Immunocytochemistry/Immunofluorescence: MAP2 Antibody (AP20) [NB600-1372] - Analysis of neurons using anti-MAP2 antibody followed by Alexa Fluor 488 goat anti-rabbit (Cat. No. A21206, green). Nuclear DNA was stained with DAPI (blue) in the merged image (right panel).

Western Blot: MAP2 Antibody (AP20) [NB600-1372] - Rat brain cortex whole cell extracts were separated on SDS-PAGE, blotted with Monoclonal Anti-MAP2 (NB120-11268) and developed using Goat Anti-Mouse IgG, Alkaline Phosphatase and NBT/BCIP substrate. (Lane A) Antibody dilution 1:500; (Lane B) Negative control (only secondary antibody).

Immunocytochemistry/Immunofluorescence: MAP2 Antibody (AP20) [NB600-1372] - DAPT, an inhibitor of Notch1 signaling, blocked the neuroprotective effects of melatonin against Abeta1-42 in hippocampal neurons. Representative images of immunofluorescent staining for MAP2. Image collected and cropped by CiteAb from the following publication (//alzres.biomedcentral.com/articles/10.1186/s13195-016-0206-x) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: MAP2 Antibody (AP20) [NB600-1372] - Human brain stained with Microtubule-Associated Protein 2a,b Ab-2 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of the neurons and dendrites.

DAPT, an inhibitor of Notch1 signaling, blocked the neuroprotective effects of melatonin against Abeta1-42 in hippocampal neurons. c) Representative images of immunofluorescent staining for MAP2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27630117), licensed under a CC-BY licence.

Um-PEA effects on neuronal viability in hippocampus of 3xTg-AD and Non-Tg mice.Evaluation of neuronal marker expression in hippocampi of 6- and 12-month-old 3xTg-AD and age-matched Non-Tg mice chronically treated with placebo (open bars) or um-PEA (black bars). (a, b) Representative fluorescent photomicrographs of microtubule-associated protein 2 (MAP2) (red) staining in the CA1 region of hippocampi at both 6 and 12 months of age, and (c) fluorescence analysis expressed as deltaF/F0. Nuclei were stained with DAPI (blue) (N = 3, in triplicate). (d) Representative western blots for MAP2 and (e) densitometric analyses normalized with beta-actin used as loading controls (N = 3, in triplicate). The results are expressed as percentage of control (Non-Tg/placebo groups). The data are presented as means +/- SEM. Statistical analysis was performed by two-way ANOVA followed by Bonferroni's multiple-comparison test (*p < 0.05; p** < 0.01). Scale bar 100 um Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41398-017-0076-4), licensed under a CC-BY licence.

Product Discontinued

MAP2 Antibody (AP20) Summary

• Immunogen: MAP2 Antibody (AP20) was developed against bovine brain microtubule associated protein 2
• Localization: Cytoskeleton
• Marker: Neuronal Dendritic Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 200
  • Western Blot 1:20 - 50
• Theoretical MW: 199 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pigeon reactivity reported in scientific literature (Braun E et al).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for MAP2 Antibody (AP20)

• DKFZp686I2148
• MAP2
• MAP-2
• MAP2A
• MAP2B
• MAP2C
• Microtubule Associated Protein 2
• microtubule-associated protein 2
• MTAP2

Background

Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).

MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).

References

1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204

2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384

3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642

4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAP2 Antibody (NB600-1372)(11)

Publications using NB600-1372

• Bronzuoli MR, Facchinetti R, Steardo L et al. Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer's Disease:In VitroandIn VivoEvidence. Oxid Med Cell Longev. 2018-01-16 [PMID: 29576849] (ICC/IF, Human)
• Zhang S, Wang P, Ren L et al. Protective effect of melatonin on soluble Abeta1-42-induced memory impairment, astrogliosis, and synaptic dysfunction via the Musashi1/Notch1/Hes1 signaling pathway in the rat hippocampus. Alzheimers Res Ther 2016-09-16 [PMID: 27630117] (IF/IHC, Rat)
• Heitz FD, Farinelli M, Mohanna S et al. The memory gene KIBRA is a bidirectional regulator of synaptic and structural plasticity in the adult brain. Neurobiol Learn Mem. 2016-08-03 [PMID: 27498008] (IF/IHC, Mouse)
• Mikaeloff F, Gelpi M, Escós A et al. Impaired Immuno-Metabolic Homeostasis and Microenvironmental Influence on Neuropsychiatric Risk in Treated HIV Infection papers.ssrn.com 2023-07-19
• Scuderi C, Bronzuoli MR, Facchinetti R, Pace L. Ultramicronized palmitoylethanolamide rescues learning and memory impairments in a triple transgenic mouse model of Alzheimer's disease by exerting anti-inflammatory and neuroprotective effects. Transl Psychiatry. 2018-01-31 [PMID: 29382825] (IF/IHC, Mouse)
• Hu C, Wang P, Zhang S et al. Neuroprotective effect of melatonin on soluble AB1-42-induced cortical neurodegeneration via Reelin-Dab1 signaling pathway. Neurol. Res. 2017-04-07 [PMID: 28385118] (ICC/IF, Rat)
• Kong HK, Wong MH, Chan HM, Lo SC. Chronic Exposure of Adult Rats to Low Doses of Methylmercury Induced a State of Metabolic Deficit in the Somatosensory Cortex. J Proteome Res. 2013-08-28 [PMID: 23984759] (WB, Rat)
• Sakurai A, Makioka K, Fukuda T et al. Accumulation of phosphorylated TDP-43 in the CNS of a patient with Cockayne syndrome. Neuropathology 2013-04-14 [PMID: 23581709] (IHC-P, Human)
• Braun E, Hotter D, Koepke L et al. A comparative assessment of aging-related NADPH diaphorase positivity in the spinal cord and medullary oblongata between pigeon and murine bioRxiv (IHC-FrFl, Pigeon)
• Maftei D, Ratano P, Fusco I et al. The prokineticin receptor antagonist PC1 rescues memory impairment induced by beta amyloid administration through the modulation of prokineticin system Neuropharmacology 2019-08-10 [PMID: 31408628] (WB, Rat)
• Kalcheva N et al. Localization of specific epitopes on human microtubule-associated protein 2. J Neurochem 63:2336-41. 1994-01-01 [PMID: 7525876]

Bioinformatics

• Gene Symbol: MAP2
• Entrez:
  • 4133(Human)
  • 17756(Mouse)
  • 25595(Rat)