MARCO Antibody (579511) [Allophycocyanin]

Mouse splenocytes were stained with Rat Anti-Mouse MARCO APC-conjugated Monoclonal Antibody (Catalog # FAB2956A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO APC-conjugated Monoclonal Antibody (Catalog # FAB2956A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 579511
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

MARCO Antibody (579511) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MARCO
  Gln70-Ser518
  Accession # Q60754
• Specificity: Detects mouse MARCO in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: MARCO
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MARCO Antibody (579511) [Allophycocyanin]

• macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2
• MARCO
• member 2
• SCARA2

Background

Mouse MARCO is a type II transmembrane glycoprotein belonging to the class A scavenger receptor family. It is constitutively expressed in subsets of macrophages found in the marginal zone of the spleen, the peritoneum, and the medullary cord of lymph nodes. The extracellular domains of MARCO form a 220 kDa disulfide-linked homotrimer on the cell surface. MARCO binds both gram positive and gram negative bacteria, as well as oxidized low-density lipoprotein. The amino acid sequence of mouse MARCO extracellular domain is 69% identical to that of human MARCO.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MARCO Antibody (FAB2956A)(5)

Publications using FAB2956A

• Núñez, SY;Trotta, A;Regge, MV;Amarilla, MS;Secchiari, F;Sierra, JM;Santilli, MC;Gantov, M;Rovegno, A;Richards, N;Ameri, C;Ríos Pita, H;Rico, L;Mieggi, M;Vitagliano, G;Blas, L;Friedrich, AD;Domaica, CI;Fuertes, MB;Zwirner, NW; Tumor-associated macrophages impair NK cell IFN-? production and contribute to tumor progression in clear cell renal cell carcinoma European journal of immunology 2024-04-06 [PMID: 38581345] (Flow Cytometry, Mouse)
• Dan Xu, Swati Bhattacharyya, Wenxia Wang, Igal Ifergan, Ming-Yi Alice Chiang Wong, Daniele Procissi et al. PLG nanoparticles target fibroblasts and MARCO+ monocytes to reverse multiorgan fibrosis JCI Insight 3/8/2022 [PMID: 35104243]
• Ruhung Wang, Rishabh Lohray, Erik Chow, Pratima Gangupantula, Loren Smith, Rockford Draper Selective Uptake of Carboxylated Multi-Walled Carbon Nanotubes by Class A Type 1 Scavenger Receptors and Impaired Phagocytosis in Alveolar Macrophages Nanomaterials (Basel) 12/3/2020 [PMID: 33287183]
• X Wu, KK Dayanand, RP Thylur, CC Norbury, DC Gowda Small molecule-based inhibition of MEK1/2 proteins dampens inflammatory responses to malaria, reduces parasite load, and mitigates pathogenic outcomes J. Biol. Chem., 2017-07-05;0(0):. 2017-07-05 [PMID: 28679535] (Flow Cytometry, Mouse)
• Wu X, Gowda N, Gowda D Phagosomal Acidification Prevents Macrophage Inflammatory Cytokine Production to Malaria, and Dendritic Cells Are the Major Source at the Early Stages of Infection: IMPLICATION FOR MALARIA PROTECTIVE IMMUNITY DEVELOPMENT. J Biol Chem, 2015-08-03;290(38):23135-47. 2015-08-03 [PMID: 26240140] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: MARCO
• Uniprot:
  • Q60754()