MCM6 Antibody (7D8) - Azide and BSA Free

Western Blot: MCM6 Antibody (7D8) [H00004175-M04] - Analysis of MCM6 expression in transfected 293T cell line by MCM6 monoclonal antibody (M04), clone 7D8. Lane 1: MCM6 transfected lysate (Predicted MW: 92.9 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MCM6 Antibody (7D8) [H00004175-M04] - Analysis of monoclonal antibody to MCM6 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: MCM6 Antibody (7D8) [H00004175-M04] - Detection limit for recombinant GST tagged MCM6 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 7D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MCM6 Antibody (7D8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MCM6 (NP_005906.2, 1 a.a. ~ 821 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLAEELIRPERNTLVVSFVDLEQFNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPLAKDFYVAFQDLPTRHKIRELTSSRIGLLTRISGQVVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYTQPNICRNPVCANRRRFLLDTNKSRFVDFQKVRIQETQAELPRGSIPRSLEVILRAEAVESAQAGDKCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLACCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNLCTSLFPTIHGNDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTSGKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQTISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED
• Specificity: This product is specific for Human MCM6 monoclonal antibody (M04), clone 7D8 [Gene ID: 4175].
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCM6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MCM6 Antibody (7D8) - Azide and BSA Free

• DNA replication licensing factor MCM6
• EC 3.6.4.12
• MCG40308
• MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S.cerevisiae)
• MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe)
• minichromosome maintenance complex component 6
• minichromosome maintenance deficient (mis5, S. pombe) 6
• minichromosome maintenance deficient 6 homolog (S. cerevisiae)
• minichromosome maintenance deficient 6 homolog
• MIS5 homolog
• Mis5
• p105MCM

Background

MCM6 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCM6 Antibody (H00004175-M04)(1)

Publication using H00004175-M04

• Polasek-Sedlackova H, Miller TCR, Krejci J et al. Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes Nature communications 2022-10-14 [PMID: 36241664] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: MCM6
• Uniprot:
  • NP_005906.2(Human)