Recombinant Human MDMX GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, AP

Recombinant Human MDMX GST (N-Term) Protein Summary

• Description: Recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-490 of Human MDM4
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQRTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLVTCFHCARRLKKAGASCPICKKEIQLVIKVFIA

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: MDM4
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 81.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human MDMX GST (N-Term) Protein

• DKFZp781B1423
• double minute 4 homolog
• Double minute 4 protein
• double minute 4, human homolog of; p53-binding protein
• HDMX
• Mdm2-like p53-binding protein
• Mdm4 p53 binding protein homolog (mouse)
• MDM4 Regulator of P53
• MDM4
• Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
• Mdm4, transformed 3T3 cell double minute 4, p53 binding protein
• MDM4-related protein 1
• MDMX
• MDMXMGC132766
• mouse double minute 4, human homolog of; p53-binding protein
• MRP1
• p53-binding protein Mdm4
• protein Mdm4
• Protein Mdmx

Background

The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2 [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for MDMX Recombinant Protein (H00004194-P01). (Showing 1 - 1 of 1 FAQ).

1. I would like to have information about the following product: MDMX Recombinant Protein (H00004194-P01) (10 ug). I read that it is the full length protein with the addition of a GST tag at its N-terminal region. You also said that the protein is not active. What does it mean? Is the protein capable of interacting with its natural partners (e.g. p53)?
   • This product is produced by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. In this unique expression system, the proteins may not always undergo the same folding and post-transnational modifications that they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays. Its a chance that they may work in your assay, but we can not provide guarantee for your mentioned use.

Bioinformatics

• Gene Symbol: MDM4