MEIS2 Antibody (1H4) - Azide and BSA Free

Western Blot: MEIS2 Antibody (1H4) [H00004212-M01] - Analysis of MEIS2 expression in transfected 293T cell line by MEIS2 monoclonal antibody (M01), clone 1H4.Lane 1: MEIS2 transfected lysate(51.1 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MEIS2 Antibody (1H4) [H00004212-M01] - Analysis of monoclonal antibody to MEIS2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: MEIS2 Antibody (1H4) [H00004212-M01] - Analysis of monoclonal antibody to MEIS2 on formalin-fixed paraffin-embedded human spleen tissue. Antibody concentration 1 ug/ml.

Western Blot: MEIS2 Antibody (1H4) [H00004212-M01] - MEIS2 monoclonal antibody (M01), clone 1H4 Analysis of MEIS2 expression in HeLa.

Western Blot: MEIS2 Antibody (1H4) [H00004212-M01] - MEIS2 monoclonal antibody (M01), clone 1H4. Analysis of MEIS2 expression in K-562.

ELISA: MEIS2 Antibody (1H4) [H00004212-M01] - Detection limit for recombinant GST tagged MEIS2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MEIS2 Antibody (1H4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MEIS2 (AAH01516, 1 a.a. ~ 381 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDGVGVPASMYGDPHAPRPIPPVHHLNHGPPLHATQHYGAHAPHPNVMPASMGSAVNDALKRDKDAIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQVRAEKPLFSSNPELDNLMIQAIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDERDGSSKSDHEELSGSSTNLADHNPSSWRDHDDATSTHSAGTPGPSSGGHASQSGDNSSEQGDGLDNSVASPGTGDDDDPDKDKKRQKKRGIFPKVATNIMRAWLFQHLTHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVSQGAAYSPEGQPMGSFVLDGQQHMGIRPAGPMSGMGMNMGMDGQWHYM
• Specificity: MEIS2 - Meis1, myeloid ecotropic viral integration site 1 homolog 2 (mouse)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MEIS2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MEIS2 Antibody (1H4) - Azide and BSA Free

• Meis (mouse) homolog 2
• Meis homeobox 2
• Meis1, myeloid ecotropic viral integration site 1 homolog 2 (mouse)
• Meis1-related gene 1
• MGC2820
• myeloid ecotropic viral integration site 1 homolog 2
• TALE homeobox protein Meis2

Background

This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEIS2 Antibody (H00004212-M01)(5)

Publications using H00004212-M01

• Shen C, Nayak A, Neitzel LR et al. The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling Nature communications 2021-09-06 [PMID: 34489457] (WB, Human)
• Fischer ES, Bohm K, Lydeard JR et al. Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide. Nature. 2014-07-16 [PMID: 25043012]
• Bjerke GA, Hyman-Walsh C, Wotton D. Cooperative transcriptional activation by klf4, meis2, and pbx1. Mol Cell Biol. 2011-07-11 [PMID: 21746878]
• Garcia-Moreno F, Pedraza M, Di Giovannantonio LG et al. A neuronal migratory pathway crossing from diencephalon to telencephalon populates amygdala nuclei. Nat Neurosci 13(6):680-9. 2010-06-01 [PMID: 20495559]
• Wright EK Jr, Page SH, Barber SA, Clements JE Prep1/Pbx2 complexes regulate CCL2 expression through the -2578 guanine polymorphism. Genes Immun 2008-07-01 [PMID: 18480829]

Bioinformatics

• Gene Symbol: MEIS2
• Entrez:
  • 4212(Human)
• OMIM:
  • 601740(Human)
• Uniprot:
  • AAH01516(Human)